EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.55 | iodoacetamide | 0.01 M, 30% inhibition | Rhodospirillum rubrum | |
4.2.1.55 | iodoacetic acid | 0.01 M, 12% inhibition | Rhodospirillum rubrum | |
4.2.1.55 | N-ethylmaleimide | 0.01 M, 23% inhibition | Rhodospirillum rubrum | |
4.2.1.55 | p-Substituted mercuribenzoate | 3 mM, 65% inhibition | Rhodospirillum rubrum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.55 | 0.00926 | - |
crotonyl-CoA | - |
Rhodospirillum rubrum | |
4.2.1.55 | 0.0263 | - |
trans-2-hexenoyl-CoA | - |
Rhodospirillum rubrum | |
4.2.1.55 | 0.08 | - |
crotonyl-[acyl-carrier protein] | - |
Rhodospirillum rubrum | |
4.2.1.55 | 0.118 | - |
Crotonylpantetheine | - |
Rhodospirillum rubrum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.17 | crotonyl-CoA + H2O | Rhodospirillum rubrum | two enoyl coenzyme A hydrases ocur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl coenzyme A to 3-hydroxybutanoyl coenzyme A. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) | (3S)-3-hydroxybutanoyl-CoA | - |
r | |
4.2.1.55 | crotonyl-CoA | Rhodospirillum rubrum | enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.17 | Rhodospirillum rubrum | - |
- |
- |
4.2.1.55 | Rhodospirillum rubrum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.55 | - |
Rhodospirillum rubrum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.55 | (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O | stereochemistry | Rhodospirillum rubrum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.55 | additional information | - |
- |
Rhodospirillum rubrum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.17 | crotonyl-CoA + H2O | two enoyl coenzyme A hydrases ocur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl coenzyme A to 3-hydroxybutanoyl coenzyme A. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) | Rhodospirillum rubrum | (3S)-3-hydroxybutanoyl-CoA | - |
r | |
4.2.1.17 | crotonyl-CoA + H2O | two enoyl coenzyme A hydrases occur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl-CoA to 3-hydroxybutanoyl-CoA. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) | Rhodospirillum rubrum | (3S)-3-hydroxybutanoyl-CoA | - |
r | |
4.2.1.55 | (3R)-3-hydroxybutanoyl-CoA | - |
Rhodospirillum rubrum | crotonyl-CoA + H2O | - |
? | |
4.2.1.55 | crotonyl-CoA | enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria | Rhodospirillum rubrum | ? | - |
? | |
4.2.1.55 | Crotonyl-CoA + H2O | - |
Rhodospirillum rubrum | (3R)-3-Hydroxybutanoyl-CoA | - |
? | |
4.2.1.55 | Crotonyl-[acyl-carrier protein] + H2O | about 50% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
4.2.1.55 | Crotonylpantetheine | about 55% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
4.2.1.55 | additional information | no activity with crotonylglutathione, acrylyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
4.2.1.55 | trans-2-Hexenoyl-CoA + H2O | about 35% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.17 | enoyl coenzyme A hydratase (L) | - |
Rhodospirillum rubrum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.55 | 8.4 | 8.6 | - |
Rhodospirillum rubrum |