BRENDA - Enzyme Database

Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris

Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.; Phytochemistry 23, 973-977 (1984)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.5.1.51
freeze-thawing or lyophilization inactivates
Citrullus lanatus subsp. vulgaris
2.5.1.51
sensitive to freezing even in the presence of 20% glycerol
Citrullus lanatus subsp. vulgaris
4.2.1.50
sensitive to freezing even in 20% glycerol
Citrullus lanatus subsp. vulgaris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.51
hydroxylamine
50 mM, almost complete inhibition
Citrullus lanatus subsp. vulgaris
2.5.1.51
KCN
10 mM, almost complete inhibition
Citrullus lanatus subsp. vulgaris
4.2.1.50
hydroxylamine
50 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
4.2.1.50
potassium cyanide
10 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.51
2.5
-
O-acetylserine
-
Citrullus lanatus subsp. vulgaris
2.5.1.51
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.51
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
2.5.1.51
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
4.2.1.50
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
4.2.1.50
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.51
O3-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
involved in biosynthesis of heterocyclic beta-substituted alanines
3-(pyrazol-1-yl)-L-alanine + acetate
-
-
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.51
Citrullus lanatus subsp. vulgaris
-
-
-
4.2.1.50
Citrullus lanatus subsp. vulgaris
-
water melon
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.51
partial
Citrullus lanatus subsp. vulgaris
4.2.1.50
-
Citrullus lanatus subsp. vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.51
additional information
barely detectable in dry seeds
Citrullus lanatus subsp. vulgaris
-
2.5.1.51
seedling
cotyledons removed, 1 week old
Citrullus lanatus subsp. vulgaris
-
4.2.1.50
seedling
-
Citrullus lanatus subsp. vulgaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.51
0.355
-
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
0.355
-
-
Citrullus lanatus subsp. vulgaris
Storage Stability
EC Number
Storage Stability
Organism
2.5.1.51
0-2°C, stable for at least 1 month
Citrullus lanatus subsp. vulgaris
4.2.1.50
0¦C, 1 month
Citrullus lanatus subsp. vulgaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.51
O-succinyl-L-serine + pyrazole
at 16% the rate of O-acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + succinate
-
-
-
?
2.5.1.51
O-sulfo-L-serine + pyrazole
at 2.5% the rate of O-acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + SO42-
-
-
-
?
2.5.1.51
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
at 11% the rate of pyrazole
5779
Citrullus lanatus subsp. vulgaris
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
2.5.1.51
O3-acetyl-L-serine + N-hydroxyurea
at 9% the rate of pyrazole
5779
Citrullus lanatus subsp. vulgaris
O-ureidoserine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
?
2.5.1.51
O3-acetyl-L-serine + pyrazole
highly specific
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
?
2.5.1.51
O3-acetyl-L-serine + pyrazole
involved in biosynthesis of heterocyclic beta-substituted alanines
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + acetate
-
-
-
-
4.2.1.50
additional information
no activity with L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + 3-amino-1,2,4-triazole
10.6% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + N-hydroxyurea
8.7% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
3-(Pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Succinyl-L-serine + pyrazole
16.4% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Sulfo-L-serine + pyrazole
2.5% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.51
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
4.2.1.50
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.51
7.3
7.4
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
7.3
7.4
O-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.51
6.5
8.5
about half-maximal activity at pH 6.5 and 8.5
Citrullus lanatus subsp. vulgaris
4.2.1.50
6.5
8.5
50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
Citrullus lanatus subsp. vulgaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.51
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate
Citrullus lanatus subsp. vulgaris
4.2.1.50
pyridoxal 5'-phosphate
1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
Citrullus lanatus subsp. vulgaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.51
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate
Citrullus lanatus subsp. vulgaris
4.2.1.50
pyridoxal 5'-phosphate
1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
Citrullus lanatus subsp. vulgaris
General Stability (protein specific)
EC Number
General Stability
Organism
2.5.1.51
freeze-thawing or lyophilization inactivates
Citrullus lanatus subsp. vulgaris
2.5.1.51
sensitive to freezing even in the presence of 20% glycerol
Citrullus lanatus subsp. vulgaris
4.2.1.50
sensitive to freezing even in 20% glycerol
Citrullus lanatus subsp. vulgaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.51
hydroxylamine
50 mM, almost complete inhibition
Citrullus lanatus subsp. vulgaris
2.5.1.51
KCN
10 mM, almost complete inhibition
Citrullus lanatus subsp. vulgaris
4.2.1.50
hydroxylamine
50 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
4.2.1.50
potassium cyanide
10 mM almost complete inhibition
Citrullus lanatus subsp. vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.51
2.5
-
O-acetylserine
-
Citrullus lanatus subsp. vulgaris
2.5.1.51
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.51
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
2.5.1.51
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
4.2.1.50
32000
-
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
4.2.1.50
58000
-
gel filtration
Citrullus lanatus subsp. vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.51
O3-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
involved in biosynthesis of heterocyclic beta-substituted alanines
3-(pyrazol-1-yl)-L-alanine + acetate
-
-
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.51
partial
Citrullus lanatus subsp. vulgaris
4.2.1.50
-
Citrullus lanatus subsp. vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.51
additional information
barely detectable in dry seeds
Citrullus lanatus subsp. vulgaris
-
2.5.1.51
seedling
cotyledons removed, 1 week old
Citrullus lanatus subsp. vulgaris
-
4.2.1.50
seedling
-
Citrullus lanatus subsp. vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.51
0.355
-
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
0.355
-
-
Citrullus lanatus subsp. vulgaris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.5.1.51
0-2°C, stable for at least 1 month
Citrullus lanatus subsp. vulgaris
4.2.1.50
0¦C, 1 month
Citrullus lanatus subsp. vulgaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.51
O-succinyl-L-serine + pyrazole
at 16% the rate of O-acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + succinate
-
-
-
?
2.5.1.51
O-sulfo-L-serine + pyrazole
at 2.5% the rate of O-acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + SO42-
-
-
-
?
2.5.1.51
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
at 11% the rate of pyrazole
5779
Citrullus lanatus subsp. vulgaris
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
2.5.1.51
O3-acetyl-L-serine + N-hydroxyurea
at 9% the rate of pyrazole
5779
Citrullus lanatus subsp. vulgaris
O-ureidoserine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
?
2.5.1.51
O3-acetyl-L-serine + pyrazole
highly specific
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
?
2.5.1.51
O3-acetyl-L-serine + pyrazole
involved in biosynthesis of heterocyclic beta-substituted alanines
5779
Citrullus lanatus subsp. vulgaris
3-(pyrazol-1-yl)-L-alanine + acetate
-
-
-
-
4.2.1.50
additional information
no activity with L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + 3-amino-1,2,4-triazole
10.6% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + N-hydroxyurea
8.7% of activity with pyrazole
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
3-(Pyrazol-1-yl)-L-alanine + acetate
-
5779
Citrullus lanatus subsp. vulgaris
-
4.2.1.50
O-Acetyl-L-serine + pyrazole
-
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Succinyl-L-serine + pyrazole
16.4% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.2.1.50
O-Sulfo-L-serine + pyrazole
2.5% of activity with O-Acetyl-L-serine
5779
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.51
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
4.2.1.50
dimer
2 * 32000, SDS-PAGE
Citrullus lanatus subsp. vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.51
7.3
7.4
-
Citrullus lanatus subsp. vulgaris
4.2.1.50
7.3
7.4
O-acetyl-L-serine + pyrazole
Citrullus lanatus subsp. vulgaris
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.51
6.5
8.5
about half-maximal activity at pH 6.5 and 8.5
Citrullus lanatus subsp. vulgaris
4.2.1.50
6.5
8.5
50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
Citrullus lanatus subsp. vulgaris