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Literature summary extracted from

  • Consevage, M.W.; Porter, R.D.; Phillips, A.T.
    Cloning and expression in Escherichia coli of histidine utilization genes from Pseudomonas putida (1985), J. Bacteriol., 162, 138-146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.68 histidine induction and activation Pseudomonas putida
3.5.1.68 N-formyl-L-glutamate induction and activation Pseudomonas putida
3.5.1.68 Urocanate induction and activation Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.68 expression in Escherichia coli RDP210 Pseudomonas putida
3.5.2.7 expression in Escherichia coli Pseudomonas putida
3.5.3.8 expression in Escherichia coli Pseudomonas putida
4.2.1.49 genomic clone, overexpressed in Escherichia coli Pseudomonas putida
4.3.1.3 expression in Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.68 N-formyl-L-glutamate + H2O Pseudomonas putida terminal reaction in the five-step pathway for histidine utilization formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O Pseudomonas spp. terminal reaction in the five-step pathway for histidine utilization formate + L-glutamate
-
 ?
3.5.3.8 N-formimidoyl-L-glutamate + H2O Pseudomonas putida
-
 L-glutamate + formamide
-
 ?
4.2.1.49 Urocanate + H2O Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.68 Pseudomonas putida
-
 ATCC 12633
-
3.5.1.68 Pseudomonas spp.
-
-
-
3.5.2.7 Pseudomonas putida
-
-
-
3.5.3.8 Pseudomonas putida
-
-
-
4.2.1.49 Pseudomonas putida
-
 formerly Pseudomonas fluorescens ATCC 12633
-
4.3.1.3 Pseudomonas putida
-
 ATCC 12633
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.68 N-formyl-L-glutamate + H2O = formate + L-glutamate regulation, induction and catabolite repression of formylglutamate amidohydrolase Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.68 N-formyl-L-glutamate + H2O terminal reaction in the five-step pathway for histidine utilization Pseudomonas putida formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O terminal reaction in the five-step pathway for histidine utilization Pseudomonas spp. formate + L-glutamate
-
 ?
3.5.3.8 N-formimidoyl-L-glutamate + H2O
-
 Pseudomonas putida L-glutamate + formamide
-
 ?
4.2.1.49 Urocanate + H2O
-
 Pseudomonas putida 4,5-Dihydro-4-oxo-5-imidazolepropanoate
-
 ?
4.2.1.49 Urocanate + H2O
-
 Pseudomonas putida ?
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas putida urocanate + NH3
-
 ?