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Literature summary extracted from
- Urocanase (Bacillus subtilis) (1971), Methods Enzymol., 17B, 51-55.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.49 | Imidazolepropionate | competitive | Bacillus subtilis |  |
| 4.2.1.49 | imidazolone propionate | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.49 | 0.077 | - |
Urocanate | - |
Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 120000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.49 | Urocanate + H2O | Bacillus subtilis | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Klebsiella aerogenes | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Bacillus subtilis SH-11 | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.49 | Bacillus subtilis | - |
derived from SB 19 | - |
| 4.2.1.49 | Bacillus subtilis SH-11 | - |
derived from SB 19 | - |
| 4.2.1.49 | Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.49 | - |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 0.278 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Klebsiella aerogenes | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis SH-11 | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis SH-11 | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 7.2 | 8 | - |
Bacillus subtilis |
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