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Literature summary extracted from

  • Sturla, L.; Bisso, A.; Zanardi, D.; Benatti, U.; De Flora, A.; Tonetti, M.
    Expression, purification and characterization of GDP-D-mannose 4,6,-dehydratase from Escherichia coli (1997), FEBS Lett., 412, 126-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.47 as fusion protein Escherichia coli

General Stability

EC Number General Stability Organism
4.2.1.47 very unstable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.47 GDP no inhibition Escherichia coli
4.2.1.47 GDP-D-glucose up to 34% inhibition Escherichia coli
4.2.1.47 GDP-L-fucose up to 73% inhibition Escherichia coli
4.2.1.47 GDP-L-fucose
-
 Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.47 0.222
-
 GDPmannose determined with fusion protein Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.47 Ca2+ activates Escherichia coli
4.2.1.47 Mg2+ activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.47 42000
-
 6 * 42000, SDS-PAGE Escherichia coli
4.2.1.47 250000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.47 GDPmannose Escherichia coli
-
 ?
-
 ?
4.2.1.47 GDPmannose Klebsiella pneumoniae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Escherichia coli
-
-
-
4.2.1.47 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.47
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.47 2.3
-
 determined with fusion protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 GDP-mannose
-
 Escherichia coli GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?
4.2.1.47 GDP-mannose
-
 Klebsiella pneumoniae GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?
4.2.1.47 GDPmannose
-
 Escherichia coli ?
-
 ?
4.2.1.47 GDPmannose
-
 Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 hexamer 6 * 42000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.47 8
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.47 NADP+
-
 Escherichia coli
4.2.1.47 NADP+
-
 Klebsiella pneumoniae