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Literature summary extracted from

  • Broschat, K.O.; Chang, S.; Serif, G.
    Purification and characterization of GDP-D-mannose 4,6 dehydratase from porcine thyroid (1985), Eur. J. Biochem., 153, 397-401.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.47 GDP 27% inhibition Sus scrofa
4.2.1.47 GDP-D-glucose 35% inhibition Sus scrofa
4.2.1.47 GDP-L-fucose 2% inhibition Sus scrofa
4.2.1.47 GTP 58% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.47 0.0038
-
 GDPmannose
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.47 41500
-
 6 * 41500, SDS-PAGE Sus scrofa
4.2.1.47 251000
-
 sedimentation equilibrium centrifugation Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.47 GDPmannose Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.47
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.47 thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.47 0.036
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
4.2.1.47 -20¦C, 2 M sucrose, 4 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 GDP-mannose
-
 Sus scrofa GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?
4.2.1.47 GDPmannose
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 hexamer 6 * 41500, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.47 37
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.47
-
-
 unstable to freezing except at high sucrose concentration Sus scrofa
4.2.1.47 4
-
 stable for 1 day, 50% loss of activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.47 7.1
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.47 6.5 8
-
 Sus scrofa