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Literature summary extracted from

  • Thompson, M.W.; Strohl, W.R.; Floss, H.G.
    Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes (1992), J. Gen. Microbiol., 138, 779-786.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.46 TDP 58% inhibition at 2 mM Streptomyces peucetius
4.2.1.46 TDP 62% inhibition at 2 mM Streptomyces sp. C5
4.2.1.46 TMP 23% inhibition at 2 mM Streptomyces peucetius
4.2.1.46 TMP 25% inhibition at 2 mM Streptomyces sp. C5
4.2.1.46 TTP 40% inhibition at 2 mM Streptomyces peucetius
4.2.1.46 TTP 37% inhibition at 2 mM Streptomyces sp. C5

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.46 0.019
-
 NAD+
-
 Streptomyces sp. C5
4.2.1.46 0.02
-
 NAD+
-
 Streptomyces peucetius
4.2.1.46 0.031
-
 dTDPglucose
-
 Streptomyces sp. C5
4.2.1.46 0.035
-
 dTDPglucose
-
 Streptomyces peucetius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.46 36000
-
 2 * 36000, gel filtration, SDS-PAGE Streptomyces peucetius
4.2.1.46 39000
-
 2 * 39000, gel filtration, SDS-PAGE Streptomyces sp. C5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.46 dTDP-glucose Streptomyces peucetius
-
 ?
-
 ?
4.2.1.46 dTDP-glucose Streptomyces sp. C5
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Streptomyces peucetius
-
-
-
4.2.1.46 Streptomyces sp. C5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.46
-
 Streptomyces peucetius
4.2.1.46
-
 Streptomyces sp. C5

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.46 0.2
-
 dTDPglucose Streptomyces peucetius
4.2.1.46 0.31
-
 dTDPglucose Streptomyces sp. C5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-glucose
-
 Streptomyces peucetius ?
-
 ?
4.2.1.46 dTDP-glucose
-
 Streptomyces sp. C5 ?
-
 ?
4.2.1.46 additional information not: ADPglucose, ADPmannose, GDPmannose, GDPglucose, UDPgalactose Streptomyces peucetius ?
-
 ?
4.2.1.46 additional information not: ADPglucose, ADPmannose, GDPmannose, GDPglucose, UDPgalactose Streptomyces sp. C5 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.46 dimer 2 * 36000, gel filtration, SDS-PAGE Streptomyces peucetius
4.2.1.46 dimer 2 * 39000, gel filtration, SDS-PAGE Streptomyces sp. C5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.46 7.6
-
 dTDPglucose Streptomyces sp. C5
4.2.1.46 7.8
-
 dTDPglucose Streptomyces peucetius

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.46 NAD+
-
 Streptomyces peucetius
4.2.1.46 NAD+
-
 Streptomyces sp. C5