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Literature summary extracted from

  • Stein, A.; Kula, M.R.; Elling, L.
    Combined preparative enzymatic synthesis of dTDP-6-deoxy-4-keto-D-glucose from dTDP and sucrose (1998), Glycoconj. J., 15, 139-145.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.46 synthesis Synthesis of dTDP-6-deoxy-4-keto-D-glucose (intermediate for synthesis of activated deoxysugars) Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
4.2.1.46 50% residual acivity after incubation at 37¦C, pH 7.0 for 2 hours, 50% residual acivity after incubation at 25¦C, pH 7.0 for 34 hours, 50% residual acivity after incubation at 25¦C, pH 8.0 for 116 hours Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.46 enzyme can be stabilized by ammonium sulfate, 2.8 M, or bovine serum albumine, 1 mg/ml Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.46 0.0072
-
 dTDPglucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.46 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.46 4.4
-
 dTDPglucose Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-glucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.46 7.7 8.3 dTDPglucose Salmonella enterica subsp. enterica serovar Typhimurium