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Literature summary extracted from

  • Dagley, S.; Jeffcoat, R.
    D-4-Deoxy-5-ketoglucarate hydro-lyase (decarboxylating) (1975), Methods Enzymol., 42C, 272-276.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.41 iodoacetamide inhibition after prolonged incubation Delftia acidovorans
4.2.1.41 iodoacetate inhibition after prolonged incubation Delftia acidovorans
4.2.1.41 p-chloromercuribenzene sulfonic acid
-
Delftia acidovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.41 118000
-
sedimentation equilibrium Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.41 5-dehydro-4-deoxy-D-glucarate Pseudomonas sp.
-
?
-
?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate Delftia acidovorans
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.41 Delftia acidovorans
-
ATCC 17455
-
4.2.1.41 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.41
-
Delftia acidovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.41 5.9
-
-
Delftia acidovorans

Storage Stability

EC Number Storage Stability Organism
4.2.1.41 -15°C, several weeks Delftia acidovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.41 5-dehydro-4-deoxy-D-glucarate D-4-deoxy-5-ketoglucarate Pseudomonas sp. 2,5-dioxopentanoate + H2O + CO2 2,5-diketovalerate ?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate D-4-deoxy-5-ketoglucarate Delftia acidovorans 2,5-dioxopentanoate + H2O + CO2 2,5-diketovalerate ?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate
-
Pseudomonas sp. ?
-
?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate
-
Delftia acidovorans ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.41 7 7.3
-
Delftia acidovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.41 6.5 8 sharp decrease in activity below pH 6.5 and above pH 8 Delftia acidovorans