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Literature summary extracted from

  • Andreesen, J.R.; Gottschalk, G.
    The occurence of a modified Entner-Doudoroff pathway in Clostridium aceticum (1969), Arch. Microbiol., 69, 160-170.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.39 sulfhydryl compounds Fe2+ and sulfhydryl compound required, cysteine, Na-thioglycollate, and glutathione equally effective Clostridium aceticum

Application

EC Number Application Comment Organism
4.2.1.39 synthesis utilization for fermentation 2-keto-3-deoxy-D-gluconate proposed, conditions for fermentation Clostridium aceticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.39 Co2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium aceticum
4.2.1.39 Fe2+ Fe2+ and sulfhydryl compound required Clostridium aceticum
4.2.1.39 Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium aceticum
4.2.1.39 Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium aceticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.39 D-gluconate Clostridium aceticum first enzyme in modified Entner-Doudoroff pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.39 Clostridium aceticum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.39 0.5
-
 cell-free extract, fructose grown Clostridium aceticum
4.2.1.39 10
-
 cell-free extract, gluconate grown Clostridium aceticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.39 D-gluconate first enzyme in modified Entner-Doudoroff pathway Clostridium aceticum ?
-
 ?
4.2.1.39 D-mannonate 16% of activity with D-gluconate Clostridium aceticum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.39 7.8
-
 enzyme assay at Clostridium aceticum