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Literature summary extracted from

  • Gottschalk, G.; Bender, R.
    D-gluconate dehydratase from Clostridium pasteurianum (1982), Methods Enzymol., 90, 283-287.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.39 sulfhydryl compounds e.g. 2-mercaptoethanol or dithiothreitol, activate and inhibit Clostridium pasteurianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.39 2-mercaptoethanol 56% inhibition at 10 mM Clostridium pasteurianum
4.2.1.39 dithiothreitol 63% inhibition at 10 mM Clostridium pasteurianum
4.2.1.39 L-cysteine 35% inhibition at 10 mM Clostridium pasteurianum
4.2.1.39 sulfhydryl compounds
-
 Clostridium pasteurianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.39 Co2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
4.2.1.39 Fe2+ at 2 mM most effective, activates and reactivates after O2 inactivation, probably two distinct sites for activation and reactivation, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
4.2.1.39 Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
4.2.1.39 Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.39 64000
-
 2 * 64000, SDS-PAGE Clostridium pasteurianum
4.2.1.39 131000
-
 sedimentation equilibrium, gel filtration Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.39 Clostridium pasteurianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.39
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.39 132
-
-
 Clostridium pasteurianum

Subunits

EC Number Subunits Comment Organism
4.2.1.39 dimer 2 * 64000, SDS-PAGE Clostridium pasteurianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.39 25
-
 enzyme assay at Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.39 7.3 7.8
-
 Clostridium pasteurianum