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Literature summary extracted from

  • Kersters, K.; De Ley, J.
    D-Gluconate dehydratase from Alcaligenes (1975), Methods Enzymol., 42, 301-304.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.39 rather labile Alcaligenes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.39 Ca2+
-
 Alcaligenes sp.
4.2.1.39 Co2+
-
 Alcaligenes sp.
4.2.1.39 EDTA 70% inhibition at 0.1 mM Alcaligenes sp.
4.2.1.39 EDTA
-
 Clostridium pasteurianum
4.2.1.39 Ni2+
-
 Alcaligenes sp.
4.2.1.39 p-chloromercuribenzoate 30% inhibition at 0.01 mM Alcaligenes sp.
4.2.1.39 p-chloromercuribenzoate
-
 Clostridium pasteurianum
4.2.1.39 Zn2+
-
 Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.39 20.8
-
 D-gluconate
-
 Alcaligenes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.39 Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
4.2.1.39 Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Alcaligenes sp.
4.2.1.39 Mg2+ 100% activation at 1 mM Alcaligenes sp.
4.2.1.39 Mn2+ 50% activation at 0.1 mM, 30% activation at 1 mM Alcaligenes sp.
4.2.1.39 Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
4.2.1.39 Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.39 270000
-
 thin-layer chromatography Alcaligenes sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.39 Agrobacterium tumefaciens
-
-
-
4.2.1.39 Alcaligenes sp.
-
 properties are conspiciously different from those of Clostridium pasteurianum
-
4.2.1.39 Alcaligenes sp. M250
-
 properties are conspiciously different from those of Clostridium pasteurianum
-
4.2.1.39 Aspergillus niger
-
-
-
4.2.1.39 Cereibacter sphaeroides
-
 i.e. Rhodopseudomonas sphaeroides
-
4.2.1.39 Clostridium pasteurianum
-
-
-
4.2.1.39 Clostridium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.39
-
 Alcaligenes sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.39 10.2
-
-
 Alcaligenes sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.39 -20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months Clostridium pasteurianum
4.2.1.39 -20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months Alcaligenes sp.
4.2.1.39 0°C, Tris-HCl buffer, pH 8.0-8.8, MgCl2, sodium-D-gluconate, 50% loss of activity after 7 days Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.39 D-fuconate 15% of activity with D-gluconate Alcaligenes sp. ?
-
 ?
4.2.1.39 D-fuconate 15% of activity with D-gluconate Alcaligenes sp. M250 ?
-
 ?
4.2.1.39 D-gluconate
-
 Clostridium pasteurianum 2-dehydro-3-deoxy-D-gluconate
-
 ?
4.2.1.39 D-gluconate
-
 Alcaligenes sp. 2-dehydro-3-deoxy-D-gluconate
-
 ?
4.2.1.39 D-gluconate
-
 Alcaligenes sp. M250 2-dehydro-3-deoxy-D-gluconate
-
 ?
4.2.1.39 D-xylonate 89% of activity with D-gluconate Alcaligenes sp. ?
-
 ?
4.2.1.39 D-xylonate 89% of activity with D-gluconate Alcaligenes sp. M250 ?
-
 ?
4.2.1.39 additional information
-
 Clostridium pasteurianum ?
-
 ?
4.2.1.39 additional information specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested Alcaligenes sp. ?
-
 ?
4.2.1.39 additional information specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested Alcaligenes sp. M250 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.39 30
-
 enzyme assay at Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.39 8.4 8.8
-
 Alcaligenes sp.