EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.34 | PCMB | 0.15 mM, complete inhibition. 80% protection by 5 mM S-citramalate | Aerobic bacterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.34 | 0.07 | - |
mesaconate | - |
Aerobic bacterium | |
4.2.1.34 | 0.11 | - |
fumarate | - |
Aerobic bacterium | |
4.2.1.34 | 0.45 | - |
S-citramalate | - |
Aerobic bacterium | |
4.2.1.34 | 0.56 | - |
S-malate | - |
Aerobic bacterium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.34 | 160000 | - |
gel filtration | Aerobic bacterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.34 | additional information | Aerobic bacterium | constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.34 | Aerobic bacterium | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.34 | 4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol | Aerobic bacterium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.34 | 7.1 | - |
- |
Aerobic bacterium |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.34 | -20°C, in presence of 20% (NH4)2SO4 or 10% glycerol in air, about 80-90% can be reactivated even after several months | Aerobic bacterium |
4.2.1.34 | 4°C, 70% loss of activity after 1 week under N2 | Aerobic bacterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.34 | (L)-citramalate | - |
Aerobic bacterium | mesaconate + H2O | - |
? | |
4.2.1.34 | (S)-malate | - |
Aerobic bacterium | fumarate + H2O | - |
? | |
4.2.1.34 | Citramalate + H2O | - |
Aerobic bacterium | Mesaconate | - |
? | |
4.2.1.34 | fumarate + H2O | - |
Aerobic bacterium | L-malate | - |
? | |
4.2.1.34 | mesaconate | - |
Aerobic bacterium | citramalate + H2O | - |
? | |
4.2.1.34 | additional information | constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate | Aerobic bacterium | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.34 | 8.5 | - |
with mesaconate as substrate | Aerobic bacterium |
4.2.1.34 | 8.7 | - |
with fumarate as substrate | Aerobic bacterium |
4.2.1.34 | 9 | - |
S-malate as substrate | Aerobic bacterium |
4.2.1.34 | 9.1 | - |
with S-citramalate as substrate | Aerobic bacterium |