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Literature summary extracted from
- Assay and purification of (+)-citramalate hydro-lyase components from Clostridium tetanomorphum (1966), J. Biol. Chem., 241, 400-408.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.34 | sulfhydryl compounds | required | Clostridium tetanomorphum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.34 | diphosphate | - |
Clostridium tetanomorphum | |
| 4.2.1.34 | phosphate | - |
Clostridium tetanomorphum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.34 | 2 | 4 | (+)-citramalate | - |
Clostridium tetanomorphum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.34 | Fe2+ | required | Clostridium tetanomorphum | |
| 4.2.1.34 | Fe2+ | Km for ferrous ammonium sulfate: 0.004 mM | Clostridium tetanomorphum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.34 | Clostridium tetanomorphum | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.2.1.34 | inactivation by exposure to oxygen, full activation by anaerobic incubation with a sulfhydryl compound for 1-2 hours at 37°C | Clostridium tetanomorphum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.34 | component I and II | Clostridium tetanomorphum |
| 4.2.1.34 | whole enzyme | Clostridium tetanomorphum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.34 | 24 | - |
- |
Clostridium tetanomorphum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.34 | (L)-citramalate | - |
Clostridium tetanomorphum | mesaconate + H2O | - |
? | |
| 4.2.1.34 | Citramalate + H2O | (+)-citramalate | Clostridium tetanomorphum | Mesaconate | - |
? | |
| 4.2.1.34 | mesaconate | - |
Clostridium tetanomorphum | citramalate + H2O | (+)-citramalate | ? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.34 | 8.2 | 8.5 | - |
Clostridium tetanomorphum |
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