EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | Cd2+ | - |
Candida maltosa | |
2.3.3.13 | Cu2+ | - |
Candida maltosa | |
2.3.3.13 | Hg2+ | - |
Candida maltosa | |
2.3.3.13 | K+ | at high concentrations, stimulation at lower concentrations | Candida maltosa | |
2.3.3.13 | leucine | competitive with respect to 2-oxo-3-methylbutanoate, non-competitive with respect to acetyl-CoA | Candida maltosa | |
2.3.3.13 | Zn2+ | - |
Candida maltosa | |
4.2.1.33 | Cd2+ | complete inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Co2+ | 3% inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Cu2+ | complete inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Fe2+ | 13% inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Hg2+ | complete inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Mg2+ | 26% inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Mn2+ | 45% inhibition at 1 mM | Candida maltosa | |
4.2.1.33 | Zn2+ | complete inhibition at 1 mM | Candida maltosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | additional information | - |
additional information | - |
Candida maltosa | |
2.3.3.13 | 0.064 | - |
acetyl-CoA | - |
Candida maltosa | |
2.3.3.13 | 0.57 | - |
2-oxo-3-methylbutanoate | - |
Candida maltosa | |
4.2.1.33 | 0.75 | - |
beta-carboxy-beta-hydroxyisocaproate | - |
Candida maltosa | |
4.2.1.33 | 1.79 | - |
citraconate | crude enzyme preparation | Candida maltosa | |
4.2.1.33 | 4.57 | - |
alpha-hydroxy-beta-carboxyisocaproate | - |
Candida maltosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | K+ | strongly dependent on the presence of monovalent cations, K+ is most effective | Candida maltosa | |
2.3.3.13 | K+ | Km: 80 mM, inhibitory at higher concentrations | Candida maltosa | |
2.3.3.13 | Li+ | can partially replace K+ in activation | Candida maltosa | |
2.3.3.13 | Rb+ | can partially replace Rb+ in activation | Candida maltosa | |
4.2.1.33 | additional information | no requirement | Candida maltosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Candida maltosa | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Candida maltosa | - |
- |
- |
4.2.1.33 | Candida maltosa | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.33 | additional information | - |
enzyme activity with citraconate 4.8-fold and 7.4-fold higher than with standard substrates | Candida maltosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Candida maltosa | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Candida maltosa | ? | - |
? | |
2.3.3.13 | 2-oxo-butanoate + acetyl-CoA + H2O | - |
Candida maltosa | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2.3.3.13 | pyruvate + acetyl-CoA + H2O | - |
Candida maltosa | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
4.2.1.33 | alpha-hydroxy-beta-carboxyisocaproate | - |
Candida maltosa | ? | - |
? | |
4.2.1.33 | beta-carboxy-beta-hydroxyisocaproate | - |
Candida maltosa | ? | - |
? | |
4.2.1.33 | citraconate | - |
Candida maltosa | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 7.5 | 8.8 | - |
Candida maltosa |
4.2.1.33 | 8.5 | - |
enzyme assay at | Candida maltosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.81 | - |
leucine | competitive, with respect to 2-oxo-3-methylbutanoate | Candida maltosa | |
2.3.3.13 | 1.3 | - |
leucine | non-competitive, with respect to acetyl-CoA | Candida maltosa | |
2.3.3.13 | 230 | - |
K+ | at high concentration | Candida maltosa |