EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.32 | overexpressed in DH5 alpha (pGS581) | Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
4.2.1.32 | cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.32 | 0.76 | - |
(R,R)-Tartrate | overexpressed strain DH5alpha(pGS581) | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.32 | 22641 | - |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |
4.2.1.32 | 32589 | - |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |
4.2.1.32 | 105000 | - |
gel filtration | Escherichia coli |
4.2.1.32 | 110500 | - |
DNA-derived values of TtdA and TtdB | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.32 | (R,R)-Tartrate | Escherichia coli | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.32 | Escherichia coli | - |
overexpressed in DH5alpha(pGS581) | - |
4.2.1.32 | Escherichia coli | - |
K12 wild-type W3110 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.32 | oxygen-labile | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.32 | -20°C, cell-extracts in air 90% loss of activity in less than 10 h | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.32 | (R,R)-Tartrate | - |
Escherichia coli | ? | - |
? | |
4.2.1.32 | (R,S)-Tartrate | in the presence of glycerol and (R,S)-tartrate under anaerobic conditions | Escherichia coli | Oxaloacetate + H2O | - |
? | |
4.2.1.32 | L-(+)-Tartrate | in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth | Escherichia coli | Oxaloacetate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.32 | tetramer | 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |