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Literature summary extracted from

  • Reaney, S.K.; Begg, C.; Bungard, S.J.; Guest J.R.
    Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of Escherichia coli and evolutioary relationship with the Class I fumarase genes (1993), J. Gen. Microbiol., 139, 1523-1530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.32 overexpressed in DH5 alpha (pGS581) Escherichia coli

General Stability

EC Number General Stability Organism
4.2.1.32 cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.32 0.76
-
 (R,R)-Tartrate overexpressed strain DH5alpha(pGS581) Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.32 22641
-
 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli
4.2.1.32 32589
-
 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli
4.2.1.32 105000
-
 gel filtration Escherichia coli
4.2.1.32 110500
-
 DNA-derived values of TtdA and TtdB Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.32 (R,R)-Tartrate Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.32 Escherichia coli
-
 overexpressed in DH5alpha(pGS581)
-
4.2.1.32 Escherichia coli
-
 K12 wild-type W3110
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.32 oxygen-labile Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.2.1.32 -20°C, cell-extracts in air 90% loss of activity in less than 10 h Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.32 (R,R)-Tartrate
-
 Escherichia coli ?
-
 ?
4.2.1.32 (R,S)-Tartrate in the presence of glycerol and (R,S)-tartrate under anaerobic conditions Escherichia coli Oxaloacetate + H2O
-
 ?
4.2.1.32 L-(+)-Tartrate in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth Escherichia coli Oxaloacetate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.32 tetramer 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli