BRENDA - Enzyme Database

Diol metabolism and diol dehydratase in Clostridium glycoliticum

Hartmanis, M.G.N.; Stadtman, T.C.; Arch. Biochem. Biophys. 245, 144-152 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.28
dithiothreitol
activates, 900% stimulation at 1 mM
Terrisporobacter glycolicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.28
EDTA
-
Terrisporobacter glycolicus
4.2.1.28
EGTA
62% inhibition at 0.05 mM
Terrisporobacter glycolicus
4.2.1.28
ethylene glycol bis -N,N,N,N-tetraacetic acid
beta-aminoethyl ether
Terrisporobacter glycolicus
4.2.1.28
hydroxylamine
-
Terrisporobacter glycolicus
4.2.1.28
Hydroxyurea
-
Terrisporobacter glycolicus
4.2.1.28
iodoacetamide
complete inhibition at 5 mM
Terrisporobacter glycolicus
4.2.1.28
o-phenanthroline
-
Terrisporobacter glycolicus
4.2.1.28
p-chloromercuriphenylsulfonic acid
complete inhibition at 0.1 mM
Terrisporobacter glycolicus
4.2.1.28
sulfhydryl compounds
-
Terrisporobacter glycolicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.28
3
-
1,2-ethanediol
-
Terrisporobacter glycolicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.28
cell membrane
associated with; intergral membrane protein
Terrisporobacter glycolicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.28
divalent cations
-
Terrisporobacter glycolicus
4.2.1.28
K+
no requirement
Terrisporobacter glycolicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.28
Terrisporobacter glycolicus
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.2.1.28
extremely O2 sensitive
Terrisporobacter glycolicus
Reaction
EC Number
Reaction
Commentary
Organism
4.2.1.28
propane-1,2-diol = propanal + H2O
radical mechanism involved
Terrisporobacter glycolicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.28
additional information
-
-
Terrisporobacter glycolicus
4.2.1.28
1.5
-
under strictly anaerobic conditions
Terrisporobacter glycolicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.28
1,2-ethanediol
-
5628
Terrisporobacter glycolicus
acetaldehyde
-
-
-
-
4.2.1.28
1,2-propanediol
-
5628
Terrisporobacter glycolicus
propanal
-
5628
Terrisporobacter glycolicus
-
4.2.1.28
additional information
specific for 1,2-ethanediol and 1,2-propanediol
5628
Terrisporobacter glycolicus
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.28
30
-
enzyme assay at
Terrisporobacter glycolicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.28
8
-
-
Terrisporobacter glycolicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.28
cobamide
not involved
Terrisporobacter glycolicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.28
dithiothreitol
activates, 900% stimulation at 1 mM
Terrisporobacter glycolicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.28
cobamide
not involved
Terrisporobacter glycolicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.28
EDTA
-
Terrisporobacter glycolicus
4.2.1.28
EGTA
62% inhibition at 0.05 mM
Terrisporobacter glycolicus
4.2.1.28
ethylene glycol bis -N,N,N,N-tetraacetic acid
beta-aminoethyl ether
Terrisporobacter glycolicus
4.2.1.28
hydroxylamine
-
Terrisporobacter glycolicus
4.2.1.28
Hydroxyurea
-
Terrisporobacter glycolicus
4.2.1.28
iodoacetamide
complete inhibition at 5 mM
Terrisporobacter glycolicus
4.2.1.28
o-phenanthroline
-
Terrisporobacter glycolicus
4.2.1.28
p-chloromercuriphenylsulfonic acid
complete inhibition at 0.1 mM
Terrisporobacter glycolicus
4.2.1.28
sulfhydryl compounds
-
Terrisporobacter glycolicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.28
3
-
1,2-ethanediol
-
Terrisporobacter glycolicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.28
cell membrane
associated with; intergral membrane protein
Terrisporobacter glycolicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.28
divalent cations
-
Terrisporobacter glycolicus
4.2.1.28
K+
no requirement
Terrisporobacter glycolicus
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.2.1.28
extremely O2 sensitive
Terrisporobacter glycolicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.28
additional information
-
-
Terrisporobacter glycolicus
4.2.1.28
1.5
-
under strictly anaerobic conditions
Terrisporobacter glycolicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.28
1,2-ethanediol
-
5628
Terrisporobacter glycolicus
acetaldehyde
-
-
-
-
4.2.1.28
1,2-propanediol
-
5628
Terrisporobacter glycolicus
propanal
-
5628
Terrisporobacter glycolicus
-
4.2.1.28
additional information
specific for 1,2-ethanediol and 1,2-propanediol
5628
Terrisporobacter glycolicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.28
30
-
enzyme assay at
Terrisporobacter glycolicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.28
8
-
-
Terrisporobacter glycolicus