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Literature summary extracted from

  • Toraya, T.
    Diol dehydrase and glycerol dehydrase, coenzyme B12-dependent isozymes (1994), Met. Ions Biol. Syst., 30, 217-254.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expressed in Escherichia coli Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 glycerol suicide inactivation in vitro, reactivation possible in presence of coenzyme, Mg2+, ATP in vivo Klebsiella pneumoniae
4.2.1.28 additional information
-
 Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 additional information
-
 additional information
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 K+ requirement for monovalent cation Klebsiella pneumoniae
4.2.1.28 K+ crystal ionic radius determines effectiveness as activator Klebsiella pneumoniae
4.2.1.28 K+ stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae
4.2.1.28 NH4+ very effective Klebsiella pneumoniae
4.2.1.28 Rb+
-
 Klebsiella pneumoniae
4.2.1.28 Tl+
-
 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 14000
-
 F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
4.2.1.28 15500
-
 F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
4.2.1.28 19173
-
 x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
4.2.1.28 23000
-
 F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
4.2.1.28 24113
-
 x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
4.2.1.28 26000
-
 1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
4.2.1.28 28071
-
 x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
4.2.1.28 60000
-
 F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
4.2.1.28 60348
-
 x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae
4.2.1.28 200000
-
 1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
4.2.1.28 220000 240000 gel filtration, sedimentation equilibrium of cell-free extracts Klebsiella pneumoniae
4.2.1.28 250000
-
 gel filtration of membrane extracts Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 additional information Klebsiella pneumoniae production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Flavobacterium sp.
-
-
-
4.2.1.28 Klebsiella pneumoniae
-
 also Klebsiella oxytoca
-
4.2.1.28 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28
-
 Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.28 propane-1,2-diol = propanal + H2O general mechanism and stereochemistry, role of coenzyme in OH-group transfer, mechanism of overall reaction, role of free radicals during reaction, studies with coenzyme analogues Klebsiella pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Salmonella enterica subsp. enterica serovar Typhimurium propanal
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?
4.2.1.28 1,2-propanediol
-
 Flavobacterium sp. propanal
-
 ?
4.2.1.28 additional information production of both electron acceptor and intermediate for fermentative oxidation of 1,2-diols Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.28 More 1 * 26000, 1 * 200000, components F, S Klebsiella pneumoniae
4.2.1.28 More F consisits of one polypeptide, S of at least four subunits, x * 60000, x * 23000, x * 15500, x * 14000, Cys, Lys, His, andArg residues essential for catalysis Klebsiella pneumoniae
4.2.1.28 oligomer x * 28071, x * 60348, x * 24113, x * 19173, estimated from nucleotide sequences of cloned genes Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide
-
 Flavobacterium sp.
4.2.1.28 cobamide slow inactivation by analogues Klebsiella pneumoniae