Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abeles, R.H.
    Dehydrations requiring vitamin B12 coenzyme (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 481-497.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 adenosylcobalamin with e-propionamide group converted to carboxylic acid, inactivation during catalytic process, analogues inhibit if added prior to coenzyme Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 220000 240000 gel filtration, sedimentation equilibrium of cell-free extracts Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 glycerol Klebsiella pneumoniae first step of anaerobic fermentation ?
-
 ?
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 glycerol Lactobacillus sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella pneumoniae
-
 formerly Aerobacter aerogenes
-
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Lactobacillus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28
-
 Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.28 propane-1,2-diol = propanal + H2O mechanism: group migration involved, stereospecificity: replacement of C2-OH-group proceeds with inversion of configuration of both isomers, mechanism of hydrogen transfer, role of cobalt: dissociation of C-Co bond of the coenzyme and Co-binding to substrate Klebsiella pneumoniae
a
4.2.1.30 glycerol = 3-hydroxypropanal + H2O mechanism of hydrogen transfer Klebsiella pneumoniae
a
4.2.1.30 glycerol = 3-hydroxypropanal + H2O mechanism of hydrogen transfer Lactobacillus sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?
4.2.1.28 glycerol first step of anaerobic fermentation Klebsiella pneumoniae ?
-
 ?
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Lactobacillus sp. propionaldehyde + H2O
-
 ?
4.2.1.30 ethylene glycol
-
 Klebsiella pneumoniae acetaldehyde + H2O
-
 ?
4.2.1.30 ethylene glycol
-
 Lactobacillus sp. acetaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Lactobacillus sp. 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 glycerol
-
 Lactobacillus sp. ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide role during catalysis: dissociation of C-Co bond of the coenzyme and Co-binding to substrate, effect of analogues, one binding site per enzyme molecule Klebsiella pneumoniae
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Lactobacillus sp.