BRENDA - Enzyme Database show

Biochemical characterization of the enzyme responsible for activated L-serine sulphydrase' activity in nematodes

Walker, J.; Barrett, J.; Exp. Parasitol. 74, 205-215 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.22
2,4-Dichlorophenol
-
Haemonchus contortus
4.2.1.22
2,4-Dichlorophenol
-
Nippostrongylus brasiliensis
4.2.1.22
alizarin
-
Haemonchus contortus
4.2.1.22
alizarin
-
Nippostrongylus brasiliensis
4.2.1.22
aminooxyacetic acid
-
Haemonchus contortus
4.2.1.22
aminooxyacetic acid
-
Nippostrongylus brasiliensis
4.2.1.22
bithionol
-
Haemonchus contortus
4.2.1.22
bithionol
-
Nippostrongylus brasiliensis
4.2.1.22
closantel
-
Haemonchus contortus
4.2.1.22
closantel
-
Nippostrongylus brasiliensis
4.2.1.22
Dichlorophene
-
Haemonchus contortus
4.2.1.22
Dichlorophene
-
Nippostrongylus brasiliensis
4.2.1.22
ellagic acid
-
Haemonchus contortus
4.2.1.22
ellagic acid
-
Nippostrongylus brasiliensis
4.2.1.22
Hexachlorophene
-
Haemonchus contortus
4.2.1.22
Hexachlorophene
-
Nippostrongylus brasiliensis
4.2.1.22
purpurogallin
-
Haemonchus contortus
4.2.1.22
purpurogallin
-
Nippostrongylus brasiliensis
4.2.1.22
quercetin
-
Haemonchus contortus
4.2.1.22
quercetin
-
Nippostrongylus brasiliensis
4.2.1.22
Rafoxanide
-
Haemonchus contortus
4.2.1.22
Rafoxanide
-
Nippostrongylus brasiliensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.22
0.21
-
L-Cys
-
Haemonchus contortus
4.2.1.22
5.58
-
2-mercaptoethanol
-
Haemonchus contortus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.22
cytosol
-
Haemonchus contortus
5829
-
4.2.1.22
cytosol
-
Nippostrongylus brasiliensis
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.22
L-Serine + homocysteine
Haemonchus contortus
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Nippostrongylus brasiliensis
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Trichostrongylus colubriformis
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Steinernema bibionis
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.22
Haemonchus contortus
-
-
-
4.2.1.22
Nippostrongylus brasiliensis
-
-
-
4.2.1.22
Steinernema bibionis
-
-
-
4.2.1.22
Trichostrongylus colubriformis
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
4.2.1.22
L-serine + L-homocysteine = L-cystathionine + H2O
sequential bisubstrate mechanism in which no product release occurs until both substrates are bound to the enzyme to form a ternary complex
Steinernema bibionis
4.2.1.22
L-serine + L-homocysteine = L-cystathionine + H2O
sequential bisubstrate mechanism in which no product release occurs until both substrates are bound to the enzyme to form a ternary complex
Trichostrongylus colubriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.22
L-Cysteine + 1-butanethiol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-butanethiol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Haemonchus contortus
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Nippostrongylus brasiliensis
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Trichostrongylus colubriformis
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-pentanethiol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-pentanethiol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + 2-mercaptoethanol
-
5597
Trichostrongylus colubriformis
HOOC-CH(NH2)-CH2-S-CH2-CH2OH + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 2-mercaptoethanol
-
5597
Steinernema bibionis
HOOC-CH(NH2)-CH2-S-CH2-CH2OH + H2S
-
-
-
-
4.2.1.22
L-Cysteine + cysteamine
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + cysteamine
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + dithioerythritol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + dithioerythritol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + dithiothreitol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + dithiothreitol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Haemonchus contortus
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Nippostrongylus brasiliensis
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Trichostrongylus colubriformis
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Trichostrongylus colubriformis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Haemonchus contortus
Cystathionine + H2O
-
5597
Haemonchus contortus
-
4.2.1.22
L-Serine + homocysteine
-
5597
Nippostrongylus brasiliensis
Cystathionine + H2O
-
5597
Nippostrongylus brasiliensis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Trichostrongylus colubriformis
Cystathionine + H2O
-
5597
Trichostrongylus colubriformis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Steinernema bibionis
Cystathionine + H2O
-
5597
Steinernema bibionis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Trichostrongylus colubriformis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Steinernema bibionis
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.22
9
-
-
Haemonchus contortus
4.2.1.22
9
-
-
Nippostrongylus brasiliensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.22
4.5
10.5
-
Nippostrongylus brasiliensis
4.2.1.22
6.5
10.5
-
Haemonchus contortus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.22
pyridoxal 5'-phosphate
requirement
Haemonchus contortus
4.2.1.22
pyridoxal 5'-phosphate
requirement
Nippostrongylus brasiliensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.22
pyridoxal 5'-phosphate
requirement
Haemonchus contortus
4.2.1.22
pyridoxal 5'-phosphate
requirement
Nippostrongylus brasiliensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.22
2,4-Dichlorophenol
-
Haemonchus contortus
4.2.1.22
2,4-Dichlorophenol
-
Nippostrongylus brasiliensis
4.2.1.22
alizarin
-
Haemonchus contortus
4.2.1.22
alizarin
-
Nippostrongylus brasiliensis
4.2.1.22
aminooxyacetic acid
-
Haemonchus contortus
4.2.1.22
aminooxyacetic acid
-
Nippostrongylus brasiliensis
4.2.1.22
bithionol
-
Haemonchus contortus
4.2.1.22
bithionol
-
Nippostrongylus brasiliensis
4.2.1.22
closantel
-
Haemonchus contortus
4.2.1.22
closantel
-
Nippostrongylus brasiliensis
4.2.1.22
Dichlorophene
-
Haemonchus contortus
4.2.1.22
Dichlorophene
-
Nippostrongylus brasiliensis
4.2.1.22
ellagic acid
-
Haemonchus contortus
4.2.1.22
ellagic acid
-
Nippostrongylus brasiliensis
4.2.1.22
Hexachlorophene
-
Haemonchus contortus
4.2.1.22
Hexachlorophene
-
Nippostrongylus brasiliensis
4.2.1.22
purpurogallin
-
Haemonchus contortus
4.2.1.22
purpurogallin
-
Nippostrongylus brasiliensis
4.2.1.22
quercetin
-
Haemonchus contortus
4.2.1.22
quercetin
-
Nippostrongylus brasiliensis
4.2.1.22
Rafoxanide
-
Haemonchus contortus
4.2.1.22
Rafoxanide
-
Nippostrongylus brasiliensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.22
0.21
-
L-Cys
-
Haemonchus contortus
4.2.1.22
5.58
-
2-mercaptoethanol
-
Haemonchus contortus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.22
cytosol
-
Haemonchus contortus
5829
-
4.2.1.22
cytosol
-
Nippostrongylus brasiliensis
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.22
L-Serine + homocysteine
Haemonchus contortus
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Nippostrongylus brasiliensis
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Trichostrongylus colubriformis
-
?
-
-
-
4.2.1.22
L-Serine + homocysteine
Steinernema bibionis
-
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.22
L-Cysteine + 1-butanethiol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-butanethiol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Haemonchus contortus
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Nippostrongylus brasiliensis
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-mercapto-2-propanol
-
5597
Trichostrongylus colubriformis
HOOC-CH(NH2)-CH2-S-CH2-CH(OH)-CH3 + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 1-pentanethiol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + 1-pentanethiol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + 2-mercaptoethanol
-
5597
Trichostrongylus colubriformis
HOOC-CH(NH2)-CH2-S-CH2-CH2OH + H2S
-
-
-
-
4.2.1.22
L-Cysteine + 2-mercaptoethanol
-
5597
Steinernema bibionis
HOOC-CH(NH2)-CH2-S-CH2-CH2OH + H2S
-
-
-
-
4.2.1.22
L-Cysteine + cysteamine
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + cysteamine
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + dithioerythritol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + dithioerythritol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + dithiothreitol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + dithiothreitol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Haemonchus contortus
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Nippostrongylus brasiliensis
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + DL-homocysteine
-
5597
Trichostrongylus colubriformis
Cystathionine + H2S
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Cysteine + monothioglycerol
-
5597
Trichostrongylus colubriformis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Haemonchus contortus
Cystathionine + H2O
-
5597
Haemonchus contortus
-
4.2.1.22
L-Serine + homocysteine
-
5597
Nippostrongylus brasiliensis
Cystathionine + H2O
-
5597
Nippostrongylus brasiliensis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Trichostrongylus colubriformis
Cystathionine + H2O
-
5597
Trichostrongylus colubriformis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Steinernema bibionis
Cystathionine + H2O
-
5597
Steinernema bibionis
-
4.2.1.22
L-Serine + homocysteine
-
5597
Haemonchus contortus
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Nippostrongylus brasiliensis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Trichostrongylus colubriformis
?
-
-
-
-
4.2.1.22
L-Serine + homocysteine
-
5597
Steinernema bibionis
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.22
9
-
-
Haemonchus contortus
4.2.1.22
9
-
-
Nippostrongylus brasiliensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.22
4.5
10.5
-
Nippostrongylus brasiliensis
4.2.1.22
6.5
10.5
-
Haemonchus contortus