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Literature summary extracted from
- Tryptophan synthase from yeast. Purification by affinity chromatography, physical properties (1974), Eur. J. Biochem., 45, 269-276.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.20 | influence of ionic strength, stable in 1 M phosphate buffer, instable in 0.1M or 0.01 M phosphate buffer | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Saccharomyces cerevisiae |  |
| 4.2.1.20 | GSH | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | indoleacetic acid | 3.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
| 4.2.1.20 | indoleacrylic acid | 0.05 mM, 50% inhibition | Saccharomyces cerevisiae | |
| 4.2.1.20 | indolebutyric acid | 7.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
| 4.2.1.20 | Indolepropionic acid | 7.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
| 4.2.1.20 | indolepyruvic acid | 2.3 mM, 50% inhibition | Saccharomyces cerevisiae | |
| 4.2.1.20 | L-cysteine | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | PCMB | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | 0.07 | - |
1-(indol-3-yl)glycerol 3-phosphate | cosubstrate L-Ser | Saccharomyces cerevisiae | |
| 4.2.1.20 | 0.16 | - |
indole | cosubstrate L-Ser | Saccharomyces cerevisiae | |
| 4.2.1.20 | 0.5 | - |
1-(indol-3-yl)glycerol 3-phosphate | no cosubstrate | Saccharomyces cerevisiae | |
| 4.2.1.20 | 5.3 | - |
L-Ser | cosubstrate indole | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.20 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.20 | - |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.20 | 4°C, 1 M potassium phosphate buffer, pH 7.6, 1 mM pyridoxal 5'-phosphate, 2 mM PMSF, 24 h, 7% loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate + L-serine | - |
Saccharomyces cerevisiae | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
| 4.2.1.20 | indole + L-serine | - |
Saccharomyces cerevisiae | L-tryptophan + H2O | - |
? | |
| 4.2.1.20 | indole-3-glycerol phosphate | - |
Saccharomyces cerevisiae | indole + D-glyceraldehyde 3-phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 6.9 | 7 | - |
Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 6 | 7.5 | - |
Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | 0.05 | - |
indoleacrylic acid | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | 2.3 | - |
indolepyruvic acid | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | 3.5 | - |
indoleacetic acid | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | 7.5 | - |
Indolepropionic acid | - |
Saccharomyces cerevisiae | |
| 4.2.1.20 | 7.5 | - |
indolebutyric acid | - |
Saccharomyces cerevisiae | |
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