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Literature summary extracted from
- Purification and characterization of 3-dehydroquinate hydrolase and shikmate oxidoreductase. Evidence for a bifunctional enzyme (1978), Biochim. Biophys. Acta, 526, 259-266.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 48000 | - |
gel filtration | Physcomitrium patens |
| 4.2.1.10 | 49500 | - |
bifunctional enzyme with activities of ec 1.1.1.25 and ec 4.2.1.10, sucrose density gradient centrifugation | Physcomitrium patens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | Physcomitrium patens | - |
3-dehydroshikimate + NADPH + H+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.25 | Physcomitrium patens | - |
enzyme complex of EC 4.2.1.10 and EC 1.1.1.25 | - |
| 4.2.1.10 | Physcomitrium patens | - |
bifunctional enzyme with activities of ec 1.1.1.25 and ec 4.2.1.10 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.25 | enzyme complex of EC 4.2.1.10 and EC 1.1.1.25 | Physcomitrium patens |
| 4.2.1.10 | bifunctional enzyme with activities of ec 1.1.1.25 and ec 4.2.1.10 | Physcomitrium patens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.10 | additional information | - |
- |
Physcomitrium patens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | - |
Physcomitrium patens | 3-dehydroshikimate + NADPH + H+ | - |
? | |
| 4.2.1.10 | 3-dehydroquinate | - |
Physcomitrium patens | 3-dehydroshikimate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.10 | monomer | 1 * 43500-48000, bifunctional enzyme with activities of ec 1.1.1.25 and ec 4.2.1.10, SDS-PAGE, gel filtration in presence of 6 M guanidine hydrochloride | Physcomitrium patens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 35 | - |
assay at | Physcomitrium patens |
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Release 2023.1 (January 2023)
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