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Literature summary extracted from
- Valyl-tRNA synthetase from Bacillus stearothermophilus. Purification and binding with the substrates L-valine and ATP (1986), Agric. Biol. Chem., 50, 2437-2444.
No PubMed abstract available
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.9 | 100000 | - |
1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
| 6.1.1.9 | 110000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.9 | Geobacillus stearothermophilus | - |
NCA 1503 | - |
| 6.1.1.9 | Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.9 | - |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.9 | 0.0045 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.9 | ATP + L-valine + tRNAVal | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-valyl-tRNAVal | - |
? |  |
| 6.1.1.9 | ATP + L-valine + tRNAVal | - |
Geobacillus stearothermophilus NCA 1503 | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.1.1.9 | monomer | 1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.9 | 52 | - |
- |
Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.9 | 8 | - |
52°C, aminoacylation | Geobacillus stearothermophilus |
| 6.1.1.9 | 8.5 | - |
30E, aminoacylation | Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.9 | 6.5 | 10 | 6.5: 30-40% of maximal activity, 10: 10% of maximal activity | Geobacillus stearothermophilus |
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