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Literature summary extracted from

  • Bachhawat, B.K.; Robinson, W.G.; Coon, M.J.
    The enzymatic cleavage of beta-hydroxy-beta-methylglutaryl coenzyme A to acetoacetate and acetyl coenzyme A (1955), J. Biol. Chem., 216, 727-736.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.4 thiols required, e.g. cysteine Sus scrofa
4.1.3.4 thiols required, e.g. cysteine Columba livia

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.4 EDTA
-
 Columba livia
4.1.3.4 EDTA
-
 Sus scrofa
4.1.3.4 Thiomalic acid
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Mg2+ required, can be substituted by Mn2+ Sus scrofa
4.1.3.4 Mg2+ required, can be substituted by Mn2+ Columba livia
4.1.3.4 Mn2+ required, can be substituted by Mg2+ Sus scrofa
4.1.3.4 Mn2+ required, can be substituted by Mg2+ Columba livia

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Columba livia
-
 pigeon
-
4.1.3.4 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4
-
 Sus scrofa
4.1.3.4
-
 Columba livia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.4 heart
-
 Sus scrofa
-
4.1.3.4 kidney
-
 Sus scrofa
-
4.1.3.4 liver
-
 Sus scrofa
-
4.1.3.4 liver
-
 Columba livia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 additional information
-
-
 Columba livia
4.1.3.4 additional information
-
 tissue distribution of activities Sus scrofa
4.1.3.4 7
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
4.1.3.4 -20°C, Tris-HCl buffer, pH7.4, 1 mM cysteine, several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA stereochemistry Sus scrofa Acetyl-CoA + acetoacetate
-
 ?
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA stereochemistry Columba livia Acetyl-CoA + acetoacetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.4 37
-
 enzyme assay at Sus scrofa
4.1.3.4 37
-
 enzyme assay at Columba livia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 7.4
-
 enzyme assay at Columba livia
4.1.3.4 10
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.4 7.4 10.5
-
 Sus scrofa