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Literature summary extracted from

  • Dekker, E.E.; Kitson, R.P.
    2-keto-4-Hydroxyglutarate aldolase: purification of the homogeneous enzyme from bovine kidney (1992), J. Biol. Chem., 267, 10507-10514.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.16 2-mercaptoethanol activates kidney enzyme Bos taurus
4.1.3.16 2-mercaptoethanol 50 mM, enhances activity 2fold Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.16 dithiodipyridine kidney enzyme Bos taurus
4.1.3.16 Hg2+ 100% inhibition at 1 microM; kidney enzyme, complete inhibition at 1 mM Bos taurus
4.1.3.16 Mn2+ 65% inhibition at 10 mM; kidney enzyme, 65% inhibition at 10 mM Bos taurus
4.1.3.16 additional information not inhibitory: mercaptoethanol, EDTA, 1,10-phenanthroline, alpha,alpha'-dipyridyl, or 8-hydroxyquinoline up to 10 mM Bos taurus
4.1.3.16 NaBH4 incubation of the enzyme with either pyruvate or glyoxylate in the presence of NaBH4 causes extensive loss of aldolase activity concomitant with stable binding of about 1.0-1.5 mol of substrate/mol of enzyme Bos taurus
4.1.3.16 p-mercuribenzoate kidney enzyme Bos taurus
4.1.3.16 sulfhydryl-reacting reagents
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 0.024
-
 L-4-hydroxy-2-oxoglutarate pH 8.3, 37°C Bos taurus
4.1.3.16 0.031
-
 D-4-hydroxy-2-oxoglutarate pH 8.3, 37°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.16 additional information no requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 35700
-
 4 * 35700, SDS-PAGE Bos taurus
4.1.3.16 36000
-
 4 * 36000, SDS-PAGE, amino acid composition, kidney enzyme Bos taurus
4.1.3.16 138000
-
 sucrose density gradient centrifugation Bos taurus
4.1.3.16 140000
-
 gel filtration Bos taurus
4.1.3.16 144000
-
 gel filtration, sucrose density gradient centrifugation, kidney enzyme Bos taurus
4.1.3.16 144500
-
 sedimentation equilibrium centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Bos taurus
-
-
-
4.1.3.16 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16
-
 Bos taurus
4.1.3.16 kidney enzyme Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.16 9.23
-
 kidney enzyme Bos taurus
4.1.3.16 9.23
-
 pH 8.4, 37°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
4.1.3.16 4¦C, Tris-HCl buffer, pH 7.4, 40% saturated with ammonium sulfate, 6 months, kidney enzyme Bos taurus
4.1.3.16 4°C, in either 50 mM Tris-HCl or potassium phosphate, pH 7.4, plus 5 mM 2-mercaptoethanol, 0.02% sodium azide, 40% saturated with ammonium sulfate, 100% of initial activity for at least 6 months Bos taurus
4.1.3.16 4°C, in either 50 mM Tris-HCl or potassium phosphate, pH 7.4, plus 5 mM 2-mercaptoethanol, 0.02% sodium azide, no substantial loss of enzymatic activity for up to 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 D-4-hydroxy-2-oxoglutarate
-
 Bos taurus pyruvate + glyoxylate
-
 r
4.1.3.16 L-4-hydroxy-2-oxoglutarate
-
 Bos taurus pyruvate + glyoxylate
-
 r
4.1.3.16 additional information enzyme shows no stereospecificity in catalyzing the aldol cleavage of the two optical isomers of 2-keto-4-hydroxyglutarate. Enzyme also catalyzes the beta-decarboxylation of oxalacetate, its decarboxylase/aldolae activity ratio is lower than that seen with the pure enzyme from either bovine liver or Escherichia coli Bos taurus ?
-
 ?
4.1.3.16 Oxaloacetate
-
 Bos taurus CO2 + pyruvate
-
 ?
4.1.3.16 pyruvate + glyoxylate
-
 Bos taurus D-4-hydroxy-2-oxoglutarate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.16 tetramer 4 * 36000, SDS-PAGE, amino acid composition, kidney enzyme Bos taurus
4.1.3.16 tetramer 4 * 35700, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.16 8.8
-
-
 Bos taurus
4.1.3.16 8.8
-
 2-keto-4-hydroxyglutarate cleavage Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.16 6.3
-
 50% of maximum activity Bos taurus
4.1.3.16 9.6
-
 60% of maximum activity Bos taurus