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Literature summary extracted from

  • Anderson, M.; Scholtz, J.M.; Schuster, S.M.
    Rat liver 4-hydroxy-2-ketoglutarate aldolase: purification and kinetic characterization (1985), Arch. Biochem. Biophys., 236, 82-97.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.16 4-hydroxy-2-oxoglutarate substrate inhibition, Ki: 35 mM Rattus norvegicus
4.1.3.16 beta-Hydroxypyruvate
-
 Rattus norvegicus
4.1.3.16 Bromopyruvate Ki: 0.018 mM Rattus norvegicus
4.1.3.16 glyoxylate Ki: 1.5 mM Rattus norvegicus
4.1.3.16 Mercaptopyruvate
-
 Rattus norvegicus
4.1.3.16 additional information double inhibitor studies Rattus norvegicus
4.1.3.16 NaBr 18% inhibition at 50 mM, only condensation Rattus norvegicus
4.1.3.16 NaF 15% inhibition at 50 mM, only condensation Rattus norvegicus
4.1.3.16 oxaloacetate competitive, Ki: 0.22 mM Rattus norvegicus
4.1.3.16 pyruvate analogs Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.16 cytoplasm 11% of total activity Rattus norvegicus 5737
-
4.1.3.16 mitochondrion 89% of total activity Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.16 additional information no requirement Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 72000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.16 additional information
-
-
 Rattus norvegicus
4.1.3.16 7.3
-
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.16 37
-
 enzyme assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.16 0.018
-
 Bromopyruvate
-
 Rattus norvegicus
4.1.3.16 0.22
-
 oxaloacetate competitive Rattus norvegicus
4.1.3.16 35
-
 4-hydroxy-2-oxoglutarate substrate inhibition Rattus norvegicus