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Literature summary extracted from

  • Nishio, K.; Kawakami, M.
    Purification and properties of alanyl-tRNA synthetase from Bombyx mori. A monomeric enzyme (1984), J. Biochem., 96, 1867-1874.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 0.0014
-
 tRNAAla
-
 Bombyx mori
6.1.1.7 0.3
-
 ATP
-
 Bombyx mori
6.1.1.7 0.8
-
 L-alanine
-
 Bombyx mori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 KCl optimum concentration: 50-100 mM Bombyx mori
6.1.1.7 Mg2+ 6-16 mM Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 115000
-
 1 * 115000, SDS-PAGE, gel filtration in 6 M guanidine-HCl Bombyx mori
6.1.1.7 116000
-
 gel filtration Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.7 silk gland posterior Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.7 2.65
-
-
 Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Bombyx mori AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 monomer 1 * 115000, SDS-PAGE, gel filtration in 6 M guanidine-HCl Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.4 8.4
-
 Bombyx mori