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Literature summary extracted from

  • Dekker, E.E.; Kobes, R.D.; Grady, S.R.
    2-keto-4-Hydroxyglutarate aldolase from bovine liver (1975), Methods Enzymol., 42C, 280-285.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.16 sulfhydryl-reacting reagents
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 additional information
-
 additional information
-
 Bos taurus
4.1.3.16 0.14
-
 (D)-4-hydroxy-alpha-ketoglutarate
-
 Bos taurus
4.1.3.16 71
-
 L-4-hydroxy-2-oxoglutarate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.16 additional information no requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 120000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.16 9.7
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
4.1.3.16 5¦C, Tris-HCl buffer, pH 7.4, two weeks, sometimes several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 L-4-hydroxy-2-oxoglutarate
-
 Bos taurus pyruvate + glyoxylate
-
 ?
4.1.3.16 additional information Keq: 11 mM Bos taurus ?
-
 ?
4.1.3.16 Oxaloacetate
-
 Bos taurus CO2 + pyruvate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.16 8.8
-
 2-keto-4-hydroxyglutarate cleavage Bos taurus