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Literature summary extracted from

  • Liu, J.Q.; Nagata, S.; Dairi, T.; Misono, H.; Shimizu, S.; Yamada, H.
    The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine (1997), Eur. J. Biochem., 245, 289-293.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.48 cloned into pUCl18, and expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.48 10
-
L-allo-threonine pH 8.0, 30°C Saccharomyces cerevisiae
4.1.2.48 55
-
L-threonine pH 8.0, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.48 41000
-
4 * 41000, SDS-PAGE Saccharomyces cerevisiae
4.1.2.48 42000
-
4 * 42000, calculated from sequence Saccharomyces cerevisiae
4.1.2.48 170000
-
gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.48 Saccharomyces cerevisiae P37303
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.48
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.48 2.5
-
pH 8.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.48 L-allo-threonine
-
Saccharomyces cerevisiae glycine + acetaldehyde
-
?
4.1.2.48 L-threonine
-
Saccharomyces cerevisiae glycine + acetaldehyde
-
r

Subunits

EC Number Subunits Comment Organism
4.1.2.48 homotetramer 4 * 41000, SDS-PAGE Saccharomyces cerevisiae
4.1.2.48 homotetramer 4 * 42000, calculated from sequence Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
4.1.2.48 GLY1
-
Saccharomyces cerevisiae
4.1.2.48 L-TA
-
Saccharomyces cerevisiae
4.1.2.48 low specificity L-TA
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.48 30
-
assay at Saccharomyces cerevisiae
4.1.2.48 55
-
aldehyde formation from L-allo-threonine Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.48 50
-
15 min, enzyme retains only 25% of activity Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.48 41
-
L-allo-threonine pH 8.0, 30°C Saccharomyces cerevisiae
4.1.2.48 43
-
L-threonine pH 8.0, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.48 8
-
assay at Saccharomyces cerevisiae
4.1.2.48 8.5 9 aldehyde formation from L-allo-threonine Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.48 6.5 10 30 min, stable Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.48 pyridoxal 5'-phosphate 2 mol pyridoxal 5'-phosphate per 4 mol of subunit Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.48 0.78
-
L-threonine pH 8.0, 30°C Saccharomyces cerevisiae
4.1.2.48 4.1
-
L-allo-threonine pH 8.0, 30°C Saccharomyces cerevisiae