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Literature summary extracted from

  • Putney, S.D.; Sauer, R.T.; Schimmmel, P.R.
    Purification and properties of alanine tRNA synthetase from Escherichia coli (1981), J. Biol. Chem., 256, 198-204.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.7 additional information K12 strain KL386 that carries the gene on a recombinant pBR322 plasmid Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 95000
-
 4 * 95000 (alpha), SDS-PAGE Escherichia coli
6.1.1.7 380000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Escherichia coli
-
 K12 strain KL386 that carries the gene on a recombinant pBR322 plasmid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.7 2.4
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 tetramer 4 * 95000 (alpha), SDS-PAGE Escherichia coli