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Literature summary extracted from

  • Wu, M.X.; Filley, S.J.; Xiong, J.; Lee, J.J.; Hill, K.A.W.
    A cysteine in the C-terminal region of alanyl-tRNA synthetase is important for aminoacylation activity (1994), Biochemistry, 33, 12260-12266.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.7 C290S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli
6.1.1.7 C665S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli
6.1.1.7 C76S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.7 5,5'-dithiobis(2-nitrobenzoate)
-
 Escherichia coli
6.1.1.7 monobromobimane
-
 Escherichia coli
6.1.1.7 p-Hydroxymercuriphenylsulfonic acid
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 0.083
-
 ATP wild-type enzyme Escherichia coli
6.1.1.7 0.095
-
 ATP Cys665Ser mtant Escherichia coli
6.1.1.7 0.12
-
 ATP Cys412Ser mutant Escherichia coli
6.1.1.7 0.18
-
 ATP Cys76Ser mutant Escherichia coli
6.1.1.7 0.21
-
 ATP Cys290Ser mutant Escherichia coli
6.1.1.7 0.23
-
 alanine Cys665Ser mutant Escherichia coli
6.1.1.7 0.33
-
 alanine mutant Cys76Ser Escherichia coli
6.1.1.7 0.34
-
 alanine wild-type Escherichia coli
6.1.1.7 0.47
-
 alanine Cys412Ser mutant Escherichia coli
6.1.1.7 0.5
-
 alanine Cys290Ser mutant Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Zinc thightly bound Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Escherichia coli
-
 wild-type and mutant enzymes with replacement ofcysteine residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.7 79
-
 ATP Cys412Ser mutant Escherichia coli
6.1.1.7 84
-
 ATP wild-type Escherichia coli
6.1.1.7 89
-
 ATP Cys665Ser mutant Escherichia coli
6.1.1.7 110
-
 ATP Cys290Ser mutant Escherichia coli
6.1.1.7 120
-
 ATP Cys76Ser mutant Escherichia coli