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Literature summary extracted from

  • Albrecht, J.; Jansen, I.; Kula, M.R.
    Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range (1993), Biotechnol. Appl. Biochem., 17, 191-203.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.46 immobilization on Eupergit (carrier consisting of macroporous beads) improves the stability considerably in the pH range below pH 5 Linum usitatissimum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.46 benzaldehyde leads to a complete and irreversible deactivation of the enzyme within 2 h incubation Linum usitatissimum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.46 43000
-
 2 * 43000, SDS-PAGE Linum usitatissimum
4.1.2.46 87000
-
 gel filtration Linum usitatissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.46 cyanide + acetone Linum usitatissimum natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogenic glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively 2-hydroxy-2-methylpropanenitrile
-
 ?
4.1.2.46 cyanide + butan-2-one Linum usitatissimum natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogen glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively (2R)-butan-2-one cyanohydrin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.46 Linum usitatissimum P93243
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.46
-
 Linum usitatissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.46 seedling
-
 Linum usitatissimum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.46 52.9
-
-
 Linum usitatissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.46 cyanide + 2-methylcyclopentanone
-
 Linum usitatissimum ?
-
 ?
4.1.2.46 cyanide + acetone
-
 Linum usitatissimum 2-hydroxy-2-methylpropanenitrile
-
 ?
4.1.2.46 cyanide + acetone natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogenic glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively Linum usitatissimum 2-hydroxy-2-methylpropanenitrile
-
 ?
4.1.2.46 cyanide + acetylcyclopropane
-
 Linum usitatissimum ?
-
 ?
4.1.2.46 cyanide + butan-2-one
-
 Linum usitatissimum (2R)-2-hydroxy-2-methylbutanenitrile 77.2% enantiomeric excess ?
4.1.2.46 cyanide + butan-2-one natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogen glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively Linum usitatissimum (2R)-butan-2-one cyanohydrin
-
 ?
4.1.2.46 cyanide + butyraldehyde
-
 Linum usitatissimum (2R)-2-hydroxypentanenitrile
-
 ?
4.1.2.46 cyanide + chloroacetone
-
 Linum usitatissimum (2R)-3-chloro-2-hydroxy-2-methylpropionitrile
-
 ?
4.1.2.46 cyanide + crotonaldehyde
-
 Linum usitatissimum (2R)-2-hydroxy-3-pentenenitrile
-
 ?
4.1.2.46 cyanide + hexan-2-one
-
 Linum usitatissimum (2R)-2-hydroxy-2-methylhexanenitrile
-
 ?
4.1.2.46 cyanide + hydroxyacetone
-
 Linum usitatissimum (2R)-1,2-dihydroxy-2-methyl-propane-3-nitrile
-
 ?
4.1.2.46 cyanide + methyl vinyl ketone
-
 Linum usitatissimum (2R)-2-hydroxy-2-methyl-3-butenenitrile
-
 ?
4.1.2.46 cyanide + pentan-2,4-dione
-
 Linum usitatissimum ?
-
 ?
4.1.2.46 cyanide + pentan-2-one
-
 Linum usitatissimum (2R)-2-hydroxy-2-methylpentanenitrile
-
 ?
4.1.2.46 cyanide + pinacolone
-
 Linum usitatissimum (2R)-2-hydroxy-2,3,3-trimethylbutyronitrile
-
 ?
4.1.2.46 cyanide + pivalaldehyde
-
 Linum usitatissimum (2R)-3,3-dimethyl-2-hydroxybutyronitrile
-
 ?
4.1.2.46 cyanide + propionaldehyde
-
 Linum usitatissimum (2R)-2-hydroxybutyronitrile
-
 ?
4.1.2.46 cyanide + pyruvic acid ethyl ester
-
 Linum usitatissimum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.46 dimer 2 * 43000, SDS-PAGE Linum usitatissimum

Synonyms

EC Number Synonyms Comment Organism
4.1.2.46 (R)-Oxynitrilase
-
 Linum usitatissimum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.46 25
-
 assay at Linum usitatissimum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.46 5.5
-
-
 Linum usitatissimum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.46 4.1 5.5 pH 4.1: about 70% of maximal activity, pH 5.5: maximal activity Linum usitatissimum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.46 4
-
 half-life: 1 h Linum usitatissimum
4.1.2.46 5
-
 immobilization on Eupergit (carrier consisting of macroporous beads) improves the stability considerably in the pH range below pH 5 Linum usitatissimum
4.1.2.46 6 11 stable Linum usitatissimum