BRENDA - Enzyme Database

Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum

Xu, L.L.; Singh, B.K.; Conn, E.E.; Arch. Biochem. Biophys. 263, 256-263 (1988) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.46
additional information
no inhibition by 10 mM 2-mercaptoethanol, 1 mM iodoacetamide or iodoacetic acid, 10 mM isobutyronitrile or isopropanol
Linum usitatissimum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.46
1.25
-
(2R)-2-hydroxy-2-methylbutanenitrile
pH 5.5, 25°C
Linum usitatissimum
4.1.2.46
2.5
-
2-hydroxy-2-methylpropanenitrile
pH 5.5, 25°C
Linum usitatissimum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.2.46
42000
-
2 * 42000, SDS-PAGE
Linum usitatissimum
4.1.2.46
82000
-
gel filtration
Linum usitatissimum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.1.2.46
2-hydroxy-2-methylpropanenitrile
Linum usitatissimum
i.e. acetone cyanohydrin
cyanide + acetone
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.1.2.46
Linum usitatissimum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.1.2.46
-
Linum usitatissimum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.2.46
shoot
-
Linum usitatissimum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.2.46
34.1
-
-
Linum usitatissimum
Storage Stability
EC Number
Storage Stability
Organism
4.1.2.46
4°C, stable for at least 45 d
Linum usitatissimum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.1.2.46
(2R)-2-hydroxy-2-methylbutanenitrile
-
5142
Linum usitatissimum
cyanide + 2-butanone
-
-
-
?
4.1.2.46
2-hydroxy-2-methylpropanenitrile
i.e. acetone cyanohydrin
5142
Linum usitatissimum
cyanide + acetone
-
-
-
?
4.1.2.46
additional information
no activity towards mandelonitrile and p-hydroxymandelonitrile
5142
Linum usitatissimum
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.2.46
dimer
2 * 42000, SDS-PAGE
Linum usitatissimum
Synonyms
EC Number
Synonyms
Commentary
Organism
4.1.2.46
Acetone cyanohydrin lyase
-
Linum usitatissimum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.46
5.5
-
-
Linum usitatissimum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.2.46
additional information
not a flavoprotein
Linum usitatissimum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.2.46
Linum usitatissimum
chromatofocusing
4.8
4.5
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.2.46
additional information
not a flavoprotein
Linum usitatissimum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.46
additional information
no inhibition by 10 mM 2-mercaptoethanol, 1 mM iodoacetamide or iodoacetic acid, 10 mM isobutyronitrile or isopropanol
Linum usitatissimum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.46
1.25
-
(2R)-2-hydroxy-2-methylbutanenitrile
pH 5.5, 25°C
Linum usitatissimum
4.1.2.46
2.5
-
2-hydroxy-2-methylpropanenitrile
pH 5.5, 25°C
Linum usitatissimum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.2.46
42000
-
2 * 42000, SDS-PAGE
Linum usitatissimum
4.1.2.46
82000
-
gel filtration
Linum usitatissimum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.1.2.46
2-hydroxy-2-methylpropanenitrile
Linum usitatissimum
i.e. acetone cyanohydrin
cyanide + acetone
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.2.46
-
Linum usitatissimum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.2.46
shoot
-
Linum usitatissimum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.2.46
34.1
-
-
Linum usitatissimum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.2.46
4°C, stable for at least 45 d
Linum usitatissimum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.1.2.46
(2R)-2-hydroxy-2-methylbutanenitrile
-
5142
Linum usitatissimum
cyanide + 2-butanone
-
-
-
?
4.1.2.46
2-hydroxy-2-methylpropanenitrile
i.e. acetone cyanohydrin
5142
Linum usitatissimum
cyanide + acetone
-
-
-
?
4.1.2.46
additional information
no activity towards mandelonitrile and p-hydroxymandelonitrile
5142
Linum usitatissimum
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.2.46
dimer
2 * 42000, SDS-PAGE
Linum usitatissimum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.46
5.5
-
-
Linum usitatissimum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.2.46
Linum usitatissimum
chromatofocusing
4.8
4.5