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Literature summary extracted from

  • Volpe, F.; Ballantine, S.P.; Delves, C.J.
    The multifunctional folic acid synthesis fas gene of Pneumocystis carinii encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase (1993), Eur. J. Biochem., 216, 449-458.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.25 pharmacology the Fas multifunctional enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase is an attractive target for chemotherapy, sin Pneumocystis carinii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 a single polypeptide, Fas protein, expressed in cultured Spodoptera frugiperda SF9 insect cells Pneumocystis carinii
2.7.6.3 multifunctional folic acid synthesis fas gene that encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, in cultured Spodoptera frugiperda SF9 insect cells Pneumocystis carinii
4.1.2.25 overexpression of the multifunctional Fas enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase in cultured Spodoptera frugiperda insect cells Pneumocystis carinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 71500
-
 polyacrylamide gel electrophoresis Pneumocystis carinii
2.7.6.3 69000
-
 calculation from multifunctional folic acid synthesis fas gene that encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase Pneumocystis carinii
4.1.2.25 71500
-
 x * 71500, Fas multifunctional enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, SDS-PAGE Pneumocystis carinii
4.1.2.25 83979
-
 x * 83979, multifunctional Fas enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, calculation from nucleotide sequence Pneumocystis carinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Pneumocystis carinii
-
 diphosphate + 7,8-dihydropteroate
-
 ?
4.1.2.25 2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine Pneumocystis carinii first enzyme in the folate synthesis pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Pneumocystis carinii
-
-
-
2.7.6.3 Pneumocystis carinii
-
 multifunctional folic acid synthesis fas gene of encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase
-
4.1.2.25 no activity in mammalia
-
-
-
4.1.2.25 Pneumocystis carinii
-
 multifunctional Fas enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Pneumocystis carinii diphosphate + 7,8-dihydropteroate
-
 ?
4.1.2.25 2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine
-
 Pneumocystis carinii 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde
-
 ?
4.1.2.25 2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine first enzyme in the folate synthesis pathway Pneumocystis carinii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.25 ? x * 83979, multifunctional Fas enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, calculation from nucleotide sequence Pneumocystis carinii
4.1.2.25 ? x * 71500, Fas multifunctional enzyme with the activity of the first three enzymes of the folate synthesis pathway: dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, SDS-PAGE Pneumocystis carinii