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Literature summary extracted from

  • Hubbard, B.K.; Koch, M.; Palmer, D.R.J.; Babbitt, P.C.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli (1998), Biochemistry, 37, 14369-14375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.20 expression in Escherichia coli BL21 Escherichia coli
4.2.1.42
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.051
-
 (R)-glycerate pH 7.5, 22°C Escherichia coli
2.7.1.165 0.061
-
 ATP pH 7.5, 22°C Escherichia coli
4.1.2.20 0.000065
-
 5-dehydro-4-deoxy-D-glucarate
-
 Escherichia coli
4.2.1.40 0.016
-
 L-idarate
-
 Escherichia coli
4.2.1.40 0.06
-
 D-glucarate
-
 Escherichia coli
4.2.1.42 0.8
-
 galactarate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.40 additional information
-
 sequence alignment Bacillus subtilis
4.2.1.40 additional information
-
 sequence alignment Escherichia coli
4.2.1.40 additional information
-
 sequence alignment Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate Escherichia coli enzymes in the (D)-glucarate/galactarate catabolic pathway ADP + 2-phospho-(R)-glycerate
-
 ?
4.1.2.20 additional information Escherichia coli glucarate catabolic enzyme ?
-
 ?
4.1.2.20 additional information Escherichia coli CR63MA glucarate catabolic enzyme ?
-
 ?
4.2.1.42 D-Galactarate Escherichia coli enzyme of D-glucarate catabolism ?
-
 ?
4.2.1.42 D-Galactarate Escherichia coli CR63MA enzyme of D-glucarate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Escherichia coli
-
-
-
4.1.2.20 Escherichia coli
-
-
-
4.2.1.40 Bacillus subtilis
-
-
-
4.2.1.40 Escherichia coli
-
-
-
4.2.1.40 Pseudomonas putida
-
-
-
4.2.1.42 Escherichia coli
-
-
-
4.2.1.42 Escherichia coli CR63MA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.20
-
 Escherichia coli
4.2.1.42
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate
-
 Escherichia coli ADP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 ATP + (R)-glycerate enzymes in the (D)-glucarate/galactarate catabolic pathway Escherichia coli ADP + 2-phospho-(R)-glycerate
-
 ?
4.1.2.20 additional information glucarate catabolic enzyme Escherichia coli ?
-
 ?
4.1.2.20 additional information glucarate catabolic enzyme Escherichia coli CR63MA ?
-
 ?
4.2.1.40 D-glucarate
-
 Bacillus subtilis 3-deoxy-L-threo-2-hexulosarate + H2O
-
 ?
4.2.1.40 D-glucarate
-
 Escherichia coli 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 D-glucarate
-
 Pseudomonas putida 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 D-glucarate
-
 Escherichia coli CR63MA 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 D-glucarate epimerization Escherichia coli D-idarate epimerization r
4.2.1.40 D-glucarate epimerization Pseudomonas putida D-idarate epimerization r
4.2.1.40 D-idarate epimerization Escherichia coli D-glucarate epimerization r
4.2.1.40 D-idarate epimerization Pseudomonas putida D-glucarate epimerization r
4.2.1.40 L-idarate
-
 Escherichia coli 3-deoxy-L-threo-2-hexulosarate i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 L-idarate
-
 Pseudomonas putida 3-deoxy-L-threo-2-hexulosarate i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 L-idarate
-
 Escherichia coli CR63MA 3-deoxy-L-threo-2-hexulosarate i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.42 D-Galactarate
-
 Escherichia coli 5-Dehydro-4-deoxy-D-glucarate + H2O
-
 ?
4.2.1.42 D-Galactarate
-
 Escherichia coli CR63MA 5-Dehydro-4-deoxy-D-glucarate + H2O
-
 ?
4.2.1.42 D-Galactarate enzyme of D-glucarate catabolism Escherichia coli ?
-
 ?
4.2.1.42 D-Galactarate enzyme of D-glucarate catabolism Escherichia coli CR63MA ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 22
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.165 2.5
-
 ATP pH 7.5, 22°C Escherichia coli
2.7.1.165 2.5
-
 (R)-glycerate pH 7.5, 22°C Escherichia coli
4.1.2.20 27.3
-
 5-dehydro-4-deoxy-D-glucarate
-
 Escherichia coli
4.2.1.40 20
-
 D-glucarate
-
 Escherichia coli
4.2.1.40 20
-
 L-idarate
-
 Escherichia coli
4.2.1.42 22
-
 galactarate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.165 7.5
-
 assay at Escherichia coli