BRENDA - Enzyme Database

2-keto-3-deoxy-D-Glucarate aldolase

Fish, D.C.; Blumenthal, H.J.; Methods Enzymol. 9, 529-534 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.20
CN-
reversed by Mg2+ and Co2+, but not by Fe2+
Escherichia coli
4.1.2.20
diphosphate
-
Escherichia coli
4.1.2.20
EDTA
-
Escherichia coli
4.1.2.20
F-
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.20
additional information
-
additional information
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.2.20
Co2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Fe2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mg2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mn2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mo2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.2.20
Escherichia coli
-
-
-
4.1.2.20
Escherichia coli CR63MA
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.2.20
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.2.20
additional information
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.2.20
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
4.1.2.20
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
4.1.2.20
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
4.1.2.20
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
4.1.2.20
pyruvate + glycolaldehyde
-
5078
Escherichia coli
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli
-
4.1.2.20
pyruvate + glycolaldehyde
-
5078
Escherichia coli CR63MA
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli CR63MA
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.20
7.7
8.6
-
Escherichia coli
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.2.20
6
7.5
stable
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.20
CN-
reversed by Mg2+ and Co2+, but not by Fe2+
Escherichia coli
4.1.2.20
diphosphate
-
Escherichia coli
4.1.2.20
EDTA
-
Escherichia coli
4.1.2.20
F-
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.20
additional information
-
additional information
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.2.20
Co2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Fe2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mg2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mn2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
4.1.2.20
Mo2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.2.20
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.2.20
additional information
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.2.20
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
4.1.2.20
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
4.1.2.20
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
4.1.2.20
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
4.1.2.20
pyruvate + glycolaldehyde
-
5078
Escherichia coli
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli
-
4.1.2.20
pyruvate + glycolaldehyde
-
5078
Escherichia coli CR63MA
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli CR63MA
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.20
7.7
8.6
-
Escherichia coli
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.2.20
6
7.5
stable
Escherichia coli