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Literature summary extracted from

  • Chiu, T.H.; Evans, K.L.; Feingold, D.S.
    L-Rhamnulose-1-phosphate aldolase (1975), Methods Enzymol., 42C, 264-269.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.19
-
 Escherichia coli

General Stability

EC Number General Stability Organism
4.1.2.19 sulfhydryl compounds stabilize Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.19 1,10-phenanthroline
-
 Escherichia coli
4.1.2.19 2,2'-dipyridyl
-
 Escherichia coli
4.1.2.19 8-hydroxyquinoline 5-sulfonic acid
-
 Escherichia coli
4.1.2.19 EDTA relieved by Co2+, Zn2+, Mn2+ or Ni2+ Escherichia coli
4.1.2.19 HgCl2 reversible Escherichia coli
4.1.2.19 L-Rhamnitol 1-phosphate
-
 Escherichia coli
4.1.2.19 Li+
-
 Escherichia coli
4.1.2.19 p-mercuribenzoate reversible Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.19 0.1
-
 L-lactaldehyde
-
 Escherichia coli
4.1.2.19 0.2
-
 glycerone phosphate
-
 Escherichia coli
4.1.2.19 0.2
-
 L-rhamnulose 1-phosphate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.19 Cs+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 K+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Na+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 NH4+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Rb+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Zn zinc metalloenzyme Escherichia coli
4.1.2.19 Zn contains 2 gatom of zinc per mol of enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.19 140000
-
 gel filtration, density gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.19 Escherichia coli
-
-
-
4.1.2.19 Escherichia coli K40
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.19
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.19 6
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.1.2.19 0-4°C, pH 7.0, 0.01 M mercaptoethanol, stable for at least 3 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.19 Glycerone phosphate + acetaldehyde
-
 Escherichia coli 5-Deoxy-L-xylulose 1-phosphate
-
 ?
4.1.2.19 glycerone phosphate + D-glyceraldehyde
-
 Escherichia coli D-sorbose 1-phosphate
-
 ?
4.1.2.19 glycerone phosphate + D-glyceraldehyde
-
 Escherichia coli K40 D-sorbose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + formaldehyde
-
 Escherichia coli D-Erythrulose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + glycolaldehyde
-
 Escherichia coli L-Xylulose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + glycolaldehyde
-
 Escherichia coli K40 L-Xylulose 1-phosphate
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate
-
 Escherichia coli Glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate
-
 Escherichia coli K40 Glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 L-Sorbose 1-phosphate
-
 Escherichia coli Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.19 L-Sorbose 1-phosphate
-
 Escherichia coli K40 Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.19 Xylulose 1-phosphate
-
 Escherichia coli Glycerone phosphate + glycoaldehyde
-
 ?
4.1.2.19 Xylulose 1-phosphate
-
 Escherichia coli K40 Glycerone phosphate + glycoaldehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.19 38.3
-
 L-rhamnulose 1-phosphate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.19 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.19 6.3 9 about 50% of maximal activity at pH 6.3 and at pH 9.0 Escherichia coli