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Literature summary extracted from
- Purification and characterization of 2-keto-3-deoxy-6-phosphogluconate aldolase from Azotobacter vinelandii: evidence that the enzyme is bifunctional towards 2-keto-4-hydroxyglutarate cleavage (1994), Biochem. Biophys. Res. Commun., 200, 459-466.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.14 | KCl | 100 mM, 67% inhibition | Azotobacter vinelandii |  |
| 4.1.2.14 | KI | 100 mM, 93% inhibition | Azotobacter vinelandii | |
| 4.1.2.14 | NaBr | 100 mM, 88% inhibition | Azotobacter vinelandii | |
| 4.1.2.14 | NaCl | 100 mM, 76% inhibition | Azotobacter vinelandii | |
| 4.1.2.14 | NaF | 100 mM, 45% inhibition | Azotobacter vinelandii | |
| 4.1.2.14 | NaI | 100 mM, 93% inhibition | Azotobacter vinelandii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.14 | 0.038 | - |
6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
Azotobacter vinelandii | |
| 4.1.3.42 | 0.039 | - |
4-hydroxy-alpha-ketoglutarate | - |
Azotobacter vinelandii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.42 | additional information | no requirement | Azotobacter vinelandii |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.14 | 24000 | - |
3 * 24000, SDS-PAGE | Azotobacter vinelandii |
| 4.1.2.14 | 70000 | - |
- |
Azotobacter vinelandii |
| 4.1.3.42 | 24000 | - |
3 * 24000, SDS-PAGE | Azotobacter vinelandii |
| 4.1.3.42 | 70000 | - |
- |
Azotobacter vinelandii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.42 | 4-hydroxy-2-oxoglutarate | Azotobacter vinelandii | involved in Entner-Doudoroff pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.14 | Azotobacter vinelandii | - |
bifunctional enzyme Ec4.1.2.14/Ec4.1.3.16 | - |
| 4.1.3.42 | Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.14 | - |
Azotobacter vinelandii |
| 4.1.3.42 | - |
Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.14 | 625 | - |
- |
Azotobacter vinelandii |
| 4.1.3.42 | 4.8 | - |
- |
Azotobacter vinelandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Azotobacter vinelandii | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.3.42 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Azotobacter vinelandii | ? | - |
? | |
| 4.1.3.42 | 4-hydroxy-2-oxoglutarate | involved in Entner-Doudoroff pathway | Azotobacter vinelandii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.14 | trimer | 3 * 24000, SDS-PAGE | Azotobacter vinelandii |
| 4.1.3.42 | trimer | 3 * 24000, SDS-PAGE | Azotobacter vinelandii |
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