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Literature summary extracted from

  • De Montigny, C.; Sygusch, J.
    Functional characterization of an extreme thermophilic class II fructose-1,6-bisphosphate aldolase (1996), Eur. J. Biochem., 241, 243-248.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.13 ethanol 10% w/v, increases activity by 24% Thermus aquaticus
4.1.2.13 Nonidet-P40 0.5% w/v, enhances activity by 34% Thermus aquaticus
4.1.2.13 Triton X-100 0.5% w/v, enhances activity by 31% Thermus aquaticus
4.1.2.13 Tween 20 0.5% w/v, enhances activity by 88% Thermus aquaticus
4.1.2.13 Tween 80 0.5% w/v, enhances activity by 39% Thermus aquaticus

General Stability

EC Number General Stability Organism
4.1.2.13 10% w/v ethanol results in 2fold reduction in thermal stability Thermus aquaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 butanol
-
 Thermus aquaticus
4.1.2.13 EDTA
-
 Thermus aquaticus
4.1.2.13 Propanol
-
 Thermus aquaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.13 Co2+ activates Thermus aquaticus
4.1.2.13 Fe2+ activates Thermus aquaticus
4.1.2.13 Zn2+ activates Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 34000
-
 4 * 34000, SDS-PAGE Thermus aquaticus
4.1.2.13 165000
-
-
 Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Thermus aquaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13
-
 Thermus aquaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.13 160
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Thermus aquaticus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 tetramer 4 * 34000, SDS-PAGE Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.13 additional information
-
 10% w/v ethanol results in 2fold reduction in thermal stability Thermus aquaticus
4.1.2.13 80
-
 2 h, stable Thermus aquaticus
4.1.2.13 90
-
 2 h, stable Thermus aquaticus
4.1.2.13 90 93 half-life: 2 h Thermus aquaticus
4.1.2.13 95
-
 half-life: 46 min Thermus aquaticus
4.1.2.13 97
-
 1 h, 80% loss of activity. Half-life: 16 min Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 6.5
-
-
 Thermus aquaticus