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Literature summary extracted from

  • Zhang, R.; Kusakabe, T.; Iwanaga, N.; Sugimoto, Y.; Kondo, K.; Takasaki, Y.; Imai, T.; Yoshida, M.; Hori, K.
    Lamprey fructose-1,6-bisphosphate aldolase: characterization of the muscle-type and non-muscle-type isozymes (1997), Arch. Biochem. Biophys., 341, 170-176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13
-
 Lethenteron camtschaticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0048
-
 D-fructose 1,6-bisphosphate non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 0.005
-
 D-fructose 1,6-bisphosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 0.016
-
 D-fructose 1,6-bisphosphate recombinant muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 0.018
-
 D-fructose 1,6-bisphosphate muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 8.3
-
 fructose 1-phosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 9.3
-
 fructose 1-phosphate muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 11
-
 fructose 1-phosphate non-muscle-type enzyme and recombinant muscle-type enzyme Lethenteron camtschaticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 40000
-
 4 * 40000, SDS-PAGE Lethenteron camtschaticum
4.1.2.13 160000
-
 glycerol density gradient centrifugation Lethenteron camtschaticum

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Lethenteron camtschaticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13
-
 Lethenteron camtschaticum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.13 liver
-
 Lethenteron camtschaticum
-
4.1.2.13 muscle
-
 Lethenteron camtschaticum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.13 5.92
-
 muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 7.01
-
 recombinant non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 17.27
-
 liver enzyme Lethenteron camtschaticum
4.1.2.13 18.5
-
 recombinat muscle-type enzyme Lethenteron camtschaticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Lethenteron camtschaticum glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate muscle-type enzyme: at 3% of the activity with fructose 1,6-diphosphate Lethenteron camtschaticum Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.13 D-Fructose 1-phosphate non-muscle-type enzyme: at about 23% of the activity with fructose 1,6-diphosphate Lethenteron camtschaticum Glycerone phosphate + D-glyceraldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 tetramer 4 * 40000, SDS-PAGE Lethenteron camtschaticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 1.38
-
 fructose 1-phosphate muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 1.55
-
 fructose 1-phosphate recombinant muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 3.6
-
 fructose 1-phosphate non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 3.65
-
 fructose 1-phosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 15.8
-
 D-fructose 1,6-bisphosphate non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 17.9
-
 D-fructose 1,6-bisphosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 46.1
-
 D-fructose 1,6-bisphosphate muscle-type enzyme Lethenteron camtschaticum
4.1.2.13 49.3
-
 D-fructose 1,6-bisphosphate recombinant muscle-type enzyme Lethenteron camtschaticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 7.5
-
-
 Lethenteron camtschaticum