BRENDA - Enzyme Database

Purification and functional characterization of the Glu-tRNAGln amidotransferase from Chlamydomonas reinhardtii

Jahn, D.; Kim, Y.C.; Ishino, Y.; Chen, M.W.; Soll, D.; J. Biol. Chem. 265, 8059-8064 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
6-diazo-5-oxonorleucine
blocking of glutamine-dependent reaction, no inhibition of ammonia-dependent reaction
Chlamydomonas reinhardtii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.7
Mg2+
required
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
63000
-
2 * 63000, SDS-PAGE
Chlamydomonas reinhardtii
6.3.5.7
120000
-
gel filtration, glycerol density gradient sedimentation
Chlamydomonas reinhardtii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.7
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.7
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492306
Chlamydomonas reinhardtii
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?
6.3.5.7
additional information
the enzyme possesses low glutaminase activity
492306
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.7
dimer
2 * 63000, SDS-PAGE
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
6-diazo-5-oxonorleucine
blocking of glutamine-dependent reaction, no inhibition of ammonia-dependent reaction
Chlamydomonas reinhardtii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.7
Mg2+
required
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
63000
-
2 * 63000, SDS-PAGE
Chlamydomonas reinhardtii
6.3.5.7
120000
-
gel filtration, glycerol density gradient sedimentation
Chlamydomonas reinhardtii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.7
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492306
Chlamydomonas reinhardtii
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
-
492306
Chlamydomonas reinhardtii
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?
6.3.5.7
additional information
the enzyme possesses low glutaminase activity
492306
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.7
dimer
2 * 63000, SDS-PAGE
Chlamydomonas reinhardtii