BRENDA - Enzyme Database

Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation

Curnow, A.W.; Hong, K.W.; Yuan, R.; Kim, S.I.; Martins, O.; Winkler, W.; Henkin, T.M.; Soll, D.; Proc. Natl. Acad. Sci. USA 94, 11819-11826 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.3.5.7
cloning of the three genes, gatC, gatA, and gatB, which constitute the transcriptional unit of the enzyme
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.7
Mg2+
required
Bacillus subtilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
10900
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
6.3.5.7
53000
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
6.3.5.7
53500
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Bacillus subtilis
disruption of this operon is lethal. Transamidation is the only pathway to Gln-tRNAGln in Bacillus subtilis. The enzyme furnishes a means for formation of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, archaea and organelles
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.7
Bacillus subtilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.7
-
Bacillus subtilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.7
0.009
-
-
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
Asn is much less effective as amide donor than glutamine
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492303
Bacillus subtilis
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
disruption of this operon is lethal. Transamidation is the only pathway to Gln-tRNAGln in Bacillus subtilis. The enzyme furnishes a means for formation of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, archaea and organelles
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
NH4Cl is much less effective as amide donor than glutamine
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.7
trimer
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.7
cloning of the three genes, gatC, gatA, and gatB, which constitute the transcriptional unit of the enzyme
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.7
Mg2+
required
Bacillus subtilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
10900
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
6.3.5.7
53000
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
6.3.5.7
53500
-
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Bacillus subtilis
disruption of this operon is lethal. Transamidation is the only pathway to Gln-tRNAGln in Bacillus subtilis. The enzyme furnishes a means for formation of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, archaea and organelles
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.7
-
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.7
0.009
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
Asn is much less effective as amide donor than glutamine
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492303
Bacillus subtilis
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
disruption of this operon is lethal. Transamidation is the only pathway to Gln-tRNAGln in Bacillus subtilis. The enzyme furnishes a means for formation of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, archaea and organelles
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
NH4Cl is much less effective as amide donor than glutamine
492303
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.7
trimer
1 * 53000 + 1 * 53500 + 1 * 10900, SDS-PAGE
Bacillus subtilis