BRENDA - Enzyme Database

Mechanistic studies of reaction coupling in Glu-tRNAGln amidotransferase

Horiuchi, K.Y.; Harpel, M.R.; Shen, L.; Luo, Y.; Rogers, K.C.; Copeland, R.A.; Biochemistry 40, 6450-6457 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.5.7
additional information
activation of glutaminase activity by ATP or ATP-gammaS together with Glu-tRNAGln, results either from an allosteric effect due simply to binding of these analogues to the enzyme or from some structural changes that attend ATP or ATP-gammaS hydrolysis
Streptococcus pyogenes
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
IC50: 2.4 mM
Streptococcus pyogenes
6.3.5.7
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
IC50: 2.4 mM
Streptococcus pyogenes
6.3.5.7
adenosine 5'-[beta,gamma-methylene]triphosphate
IC50: 2.3 mM
Streptococcus pyogenes
6.3.5.7
ADP
IC50: 0.026 mM
Streptococcus pyogenes
6.3.5.7
ATP-gammaS
IC50: 0.19 mM
Streptococcus pyogenes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.0002
-
L-glutamyl-tRNAGlu
-
Streptococcus pyogenes
6.3.5.7
0.0159
-
Gln
-
Streptococcus pyogenes
6.3.5.7
0.117
-
ATP
-
Streptococcus pyogenes
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Streptococcus pyogenes
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.7
Streptococcus pyogenes
-
recombinant enzyme expressed in Escherichia coli
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
492302
Streptococcus pyogenes
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
492302
Streptococcus pyogenes
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492302
Streptococcus pyogenes
?
6.3.5.7
ATP-gammaS + Glu-tRNAGln + L-glutamine
-
492302
Streptococcus pyogenes
? + phosphate + Gln-tRNAGln + L-glutamate
-
492302
Streptococcus pyogenes
?
6.3.5.7
additional information
in absence of the amido acceptor, Glu-tRNAGln, the enzyme has basal glutaminase activity that is unaffected by ATP
492302
Streptococcus pyogenes
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.51
-
Gln
-
Streptococcus pyogenes
6.3.5.7
0.59
-
ATP
-
Streptococcus pyogenes
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
6.3.5.7
0.026
-
IC50: 0.026 mM
Streptococcus pyogenes
ADP
6.3.5.7
0.19
-
IC50: 0.19 mM
Streptococcus pyogenes
ATP-gammaS
6.3.5.7
2.3
-
IC50: 2.3 mM
Streptococcus pyogenes
adenosine 5'-[beta,gamma-methylene]triphosphate
6.3.5.7
2.4
-
IC50: 2.4 mM
Streptococcus pyogenes
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
6.3.5.7
2.4
-
IC50: 2.4 mM
Streptococcus pyogenes
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.5.7
additional information
activation of glutaminase activity by ATP or ATP-gammaS together with Glu-tRNAGln, results either from an allosteric effect due simply to binding of these analogues to the enzyme or from some structural changes that attend ATP or ATP-gammaS hydrolysis
Streptococcus pyogenes
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
6.3.5.7
0.026
-
IC50: 0.026 mM
Streptococcus pyogenes
ADP
6.3.5.7
0.19
-
IC50: 0.19 mM
Streptococcus pyogenes
ATP-gammaS
6.3.5.7
2.3
-
IC50: 2.3 mM
Streptococcus pyogenes
adenosine 5'-[beta,gamma-methylene]triphosphate
6.3.5.7
2.4
-
IC50: 2.4 mM
Streptococcus pyogenes
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
6.3.5.7
2.4
-
IC50: 2.4 mM
Streptococcus pyogenes
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
IC50: 2.4 mM
Streptococcus pyogenes
6.3.5.7
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
IC50: 2.4 mM
Streptococcus pyogenes
6.3.5.7
adenosine 5'-[beta,gamma-methylene]triphosphate
IC50: 2.3 mM
Streptococcus pyogenes
6.3.5.7
ADP
IC50: 0.026 mM
Streptococcus pyogenes
6.3.5.7
ATP-gammaS
IC50: 0.19 mM
Streptococcus pyogenes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.0002
-
L-glutamyl-tRNAGlu
-
Streptococcus pyogenes
6.3.5.7
0.0159
-
Gln
-
Streptococcus pyogenes
6.3.5.7
0.117
-
ATP
-
Streptococcus pyogenes
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Streptococcus pyogenes
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
492302
Streptococcus pyogenes
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis
492302
Streptococcus pyogenes
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
492302
Streptococcus pyogenes
?
6.3.5.7
ATP-gammaS + Glu-tRNAGln + L-glutamine
-
492302
Streptococcus pyogenes
? + phosphate + Gln-tRNAGln + L-glutamate
-
492302
Streptococcus pyogenes
?
6.3.5.7
additional information
in absence of the amido acceptor, Glu-tRNAGln, the enzyme has basal glutaminase activity that is unaffected by ATP
492302
Streptococcus pyogenes
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.51
-
Gln
-
Streptococcus pyogenes
6.3.5.7
0.59
-
ATP
-
Streptococcus pyogenes