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Literature summary extracted from

  • Pelchat, M.; Lacoste, L.; Yang, F.; Lapointe, J.
    Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli (1998), Can. J. Microbiol., 44, 378-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.17 gene gltX, expression in Escherichia coli is lethal, but a modulated enzyme, lacking a part of the tRNA acceptor stem binding domain, can be recombinantly expressed in Escherichia coli DH5alpha, overexpression in Bacillus subtilis Bacillus subtilis
6.1.1.24 the gene is cloned with its sigmaA promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and bacillus subtilis. Transformation of Bacillus subtilis with this recombinant plasmidleads to a 30fold increase of glutamyl-tRNA synthetase specific activity in crude extracts. Transformation of Escherichia coli with this plasmid gives no recombinants. The presence of Bacillus subtilis glutamyl-tRNA synthetase is lethal for Escherichia coli, probably because this enzyme glutamylates tRNA1Gln in vivo as it does in vitro Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.17 55000
-
 x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln Bacillus subtilis
-
 AMP + diphosphate + L-glutamyl-tRNAGln
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Bacillus subtilis
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information Bacillus subtilis the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Bacillus subtilis
-
-
-
6.1.1.24 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17 50fold, recombinant enzyme from overexpression in Bacillus subtilis, to homogeneity Bacillus subtilis
6.1.1.24
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.17 0.16
-
 purified recombinant enzyme expressed in Bacillus subtilis Bacillus subtilis
6.1.1.24 0.16
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln
-
 Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGln
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln Bacillus subtilis ?
-
 ?
6.1.1.24 ATP + L-glutamate + tRNAGln
-
 Bacillus subtilis AMP + diphosphate + Glu-tRNAGln
-
 ?
6.1.1.24 ATP + L-glutamate + tRNAGlu
-
 Bacillus subtilis AMP + diphosphate + Glu-tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 ? x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
 Bacillus subtilis
6.1.1.17 Glutamyl-tRNA synthetase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.17 37
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
 Bacillus subtilis