BRENDA - Enzyme Database

A single glutamyl-tRNA synthetase aminoacylates tRNAGlu and tRNAGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro

Lapointe, J.; Duplain, L.; Proulx, M.; J. Bacteriol. 165, 88-93 (1986)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.24
0.00045
-
tRNAGln
homologous tRNAGln, in absence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.00065
-
tRNAGln
homologous tRNAGln, in presence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.00083
-
tRNAGlu
homologous tRNAGlu, in absence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.0014
-
tRNAGlu
homologous tRNAGlu, in presence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.0015
-
tRNAGln
tRNAGln
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGlx
Bacillus subtilis
the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlx
-
Bacillus subtilis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.24
Bacillus subtilis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
the enzyme aminoacylates tRNAGLU and tRNASGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGln
-
-
-
-
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme aminoacylates tRNAGlu and tRNAsGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro, not tRNA2Gln from Escherichia coli or tRNAGlu from Escherichia coli
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlu
-
492293
Bacillus subtilis
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme interacts with the G64-C50 or G64-U50 in the Tpsi stem of its tRNA substrate
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlu
-
492293
Bacillus subtilis
?
6.1.1.24
ATP + L-glutamate + tRNAGlx
the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlx
-
492293
Bacillus subtilis
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.24
0.04
-
tRNAGln
homologous tRNAGln
Bacillus subtilis
6.1.1.24
0.05
-
tRNAGlu
homologous tRNAGlu
Bacillus subtilis
6.1.1.24
0.07
-
tRNAGln
tRNAGln from E. coli
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.24
0.00045
-
tRNAGln
homologous tRNAGln, in absence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.00065
-
tRNAGln
homologous tRNAGln, in presence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.00083
-
tRNAGlu
homologous tRNAGlu, in absence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.0014
-
tRNAGlu
homologous tRNAGlu, in presence of regulatory factor beta
Bacillus subtilis
6.1.1.24
0.0015
-
tRNAGln
tRNAGln
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGlx
Bacillus subtilis
the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlx
-
Bacillus subtilis
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
the enzyme aminoacylates tRNAGLU and tRNASGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGln
-
-
-
-
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme aminoacylates tRNAGlu and tRNAsGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro, not tRNA2Gln from Escherichia coli or tRNAGlu from Escherichia coli
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlu
-
492293
Bacillus subtilis
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme interacts with the G64-C50 or G64-U50 in the Tpsi stem of its tRNA substrate
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlu
-
492293
Bacillus subtilis
?
6.1.1.24
ATP + L-glutamate + tRNAGlx
the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis
492293
Bacillus subtilis
AMP + diphosphate + Glu-tRNAGlx
-
492293
Bacillus subtilis
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.24
0.04
-
tRNAGln
homologous tRNAGln
Bacillus subtilis
6.1.1.24
0.05
-
tRNAGlu
homologous tRNAGlu
Bacillus subtilis
6.1.1.24
0.07
-
tRNAGln
tRNAGln from E. coli
Bacillus subtilis