BRENDA - Enzyme Database

Charging of both, plastidial tRNAgln and tRNAglu with glutamate and subsequent amidation of the misacylated tRNAgln by a glutamyl-tRNA amidotransferase in the unicellular green alga Scenedesmus obliquus, mutant C-2A'

Vothknecht, U.C.; Doernemann, D.; Z. Naturforsch. C 50, 789-795 (1995)
No PubMed abstract available

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.24
plastid
-
Tetradesmus obliquus
9536
-
6.3.5.7
chloroplast
-
Tetradesmus obliquus
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGlx
Tetradesmus obliquus
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
AMP + diphosphate + Glu-tRNAGlx
-
Tetradesmus obliquus
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Tetradesmus obliquus
the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.24
Tetradesmus obliquus
-
mutant C-2A
-
6.3.5.7
Tetradesmus obliquus
-
mutant C-2A
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.24
-
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGln
-
492291
Tetradesmus obliquus
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGlu
-
492291
Tetradesmus obliquus
?
6.1.1.24
ATP + L-glutamate + tRNAGlx
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGlx
-
492291
Tetradesmus obliquus
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.24
plastid
-
Tetradesmus obliquus
9536
-
6.3.5.7
chloroplast
-
Tetradesmus obliquus
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGlx
Tetradesmus obliquus
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
AMP + diphosphate + Glu-tRNAGlx
-
Tetradesmus obliquus
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
Tetradesmus obliquus
the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.24
-
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.24
ATP + L-glutamate + tRNAGln
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGln
-
492291
Tetradesmus obliquus
?
6.1.1.24
ATP + L-glutamate + tRNAGlu
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGlu
-
492291
Tetradesmus obliquus
?
6.1.1.24
ATP + L-glutamate + tRNAGlx
the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
AMP + diphosphate + Glu-tRNAGlx
-
492291
Tetradesmus obliquus
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis
492291
Tetradesmus obliquus
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?