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Literature summary extracted from

  • Vothknecht, U.C.; Doernemann, D.
    Charging of both, plastidial tRNAgln and tRNAglu with glutamate and subsequent amidation of the misacylated tRNAgln by a glutamyl-tRNA amidotransferase in the unicellular green alga Scenedesmus obliquus, mutant C-2A' (1995), Z. Naturforsch. C, 50, 789-795.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.24 plastid
-
 Tetradesmus obliquus 9536
-
6.3.5.7 chloroplast
-
 Tetradesmus obliquus 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.24 ATP + L-glutamate + tRNAGlx Tetradesmus obliquus the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis AMP + diphosphate + Glu-tRNAGlx
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine Tetradesmus obliquus the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.24 Tetradesmus obliquus
-
 mutant C-2A
-
6.3.5.7 Tetradesmus obliquus
-
 mutant C-2A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.24
-
 Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.24 ATP + L-glutamate + tRNAGln the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis Tetradesmus obliquus AMP + diphosphate + Glu-tRNAGln
-
 ?
6.1.1.24 ATP + L-glutamate + tRNAGlu the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis Tetradesmus obliquus AMP + diphosphate + Glu-tRNAGlu
-
 ?
6.1.1.24 ATP + L-glutamate + tRNAGlx the enzyme is capable of mischarging plastidal tRNAGln from barley with glutamate as well as regularly charges the plastidal tRNAGlu from Scenedesmus. The mischarged glutamyl-tRNAGln is subsequently amidated by glutamyl-tRNA amidotransferase to form the glutaminyl-tRNAGln required for plastidal protein biosynthesis Tetradesmus obliquus AMP + diphosphate + Glu-tRNAGlx
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine the enzyme produces Gln-tRNAGln required for plastidal protein biosynthesis Tetradesmus obliquus ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?