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Literature summary extracted from
- Overexpression, purification, and characterization of isochorismate synthase (EnrC), the first enzyme involved in the biosynthesis of Enterobactin from Chorismate (1990), Biochemistry, 29, 1417-1425.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.4.2 | - |
Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.4.2 | 0.005 | - |
isochorismate | - |
Escherichia coli |  |
| 5.4.4.2 | 0.014 | - |
chorismate | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 42000 | - |
isochorismate synthase involved in menaquinone biosynthesis, EntC, gel filtration | Escherichia coli |
| 5.4.4.2 | 43000 | - |
1 * 43000, isochorismate synthase involved in menaquinone biosynthesis, EntC, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.4.2 | Chorismate | Escherichia coli | the first enzyme involved in the biosynthesis of the powerful iron-chelating agent enterobactin | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.4.2 | Escherichia coli | - |
isochorismate synthase involved in menaquinone biosynthesis, EntC | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.4.2 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.4.2 | Chorismate | r, isochorismate synthase involved in menaquinone biosynthesis, EntC | Escherichia coli | Isochorismate | - |
? | |
| 5.4.4.2 | Chorismate | the first enzyme involved in the biosynthesis of the powerful iron-chelating agent enterobactin | Escherichia coli | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.4.2 | monomer | 1 * 43000, isochorismate synthase involved in menaquinone biosynthesis, EntC, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.4.2 | 1.8 | - |
isochorismate | - |
Escherichia coli | |
| 5.4.4.2 | 2.88 | - |
chorismate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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