BRENDA - Enzyme Database show

A novel enzyme, maltose 1-epimerase from Lactobacillus brevis IFO 3345

Shirokane, Y.; Suzuki, M.; FEBS Lett. 367, 177-179 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.3.21
Cu2+
-
Lactobacillus brevis
5.1.3.21
Hg2+
-
Lactobacillus brevis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.3.21
2.2
-
beta-maltose
-
Lactobacillus brevis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.3.21
43000
-
gel filtration
Lactobacillus brevis
5.1.3.21
45000
-
1 * 45000, SDS-PAGE
Lactobacillus brevis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.1.3.21
Lactobacillus brevis
-
IFO 3345
-
Purification (Commentary)
EC Number
Commentary
Organism
5.1.3.21
-
Lactobacillus brevis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.1.3.21
1710
-
-
Lactobacillus brevis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.3.21
alpha-D-glucose
at 26% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-D-glucose
-
-
-
r
5.1.3.21
alpha-lactose
at 5% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-lactose
-
-
-
?
5.1.3.21
beta-cellobiose
at 4% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-cellobiose
-
-
-
?
5.1.3.21
beta-D-glucose
at 27% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-D-glucose
-
-
-
r
5.1.3.21
beta-Maltose
-
492266
Lactobacillus brevis
alpha-Maltose
-
492266
Lactobacillus brevis
r
Subunits
EC Number
Subunits
Commentary
Organism
5.1.3.21
monomer
1 * 45000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.1.3.21
40
-
-
Lactobacillus brevis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.1.3.21
45
-
10 min, 50 mM phosphate buffer, stable
Lactobacillus brevis
5.1.3.21
60
-
10 min, 50 mM phosphate buffer, 50% loss of activity
Lactobacillus brevis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.3.21
6.5
7
-
Lactobacillus brevis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.1.3.21
5
8.5
more than 80% of activity in the pH-range pH 5.0-8.5
Lactobacillus brevis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.3.21
Cu2+
-
Lactobacillus brevis
5.1.3.21
Hg2+
-
Lactobacillus brevis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.3.21
2.2
-
beta-maltose
-
Lactobacillus brevis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.3.21
43000
-
gel filtration
Lactobacillus brevis
5.1.3.21
45000
-
1 * 45000, SDS-PAGE
Lactobacillus brevis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.3.21
-
Lactobacillus brevis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.1.3.21
1710
-
-
Lactobacillus brevis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.3.21
alpha-D-glucose
at 26% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-D-glucose
-
-
-
r
5.1.3.21
alpha-lactose
at 5% of the activity with beta-maltose
492266
Lactobacillus brevis
beta-lactose
-
-
-
?
5.1.3.21
beta-cellobiose
at 4% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-cellobiose
-
-
-
?
5.1.3.21
beta-D-glucose
at 27% of the activity with beta-maltose
492266
Lactobacillus brevis
alpha-D-glucose
-
-
-
r
5.1.3.21
beta-Maltose
-
492266
Lactobacillus brevis
alpha-Maltose
-
492266
Lactobacillus brevis
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.1.3.21
monomer
1 * 45000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.1.3.21
40
-
-
Lactobacillus brevis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.1.3.21
45
-
10 min, 50 mM phosphate buffer, stable
Lactobacillus brevis
5.1.3.21
60
-
10 min, 50 mM phosphate buffer, 50% loss of activity
Lactobacillus brevis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.3.21
6.5
7
-
Lactobacillus brevis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.1.3.21
5
8.5
more than 80% of activity in the pH-range pH 5.0-8.5
Lactobacillus brevis