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Literature summary extracted from

  • Saito, T.; Abe, H.; Yamane, H.; Sakurai, A.; Murofushi, N.; Takio, K.; Takahashi, N.; Kamiya, Y.
    Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin (1995), Plant Physiol., 109, 1239-1245.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.19 Ba2+
-
 Cucurbita maxima
4.2.3.19 Ca2+
-
 Cucurbita maxima
4.2.3.19 Cu2+
-
 Cucurbita maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.19 0.0004
-
 copalyl diphosphate
-
 Cucurbita maxima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.19 Co2+ can partially replace Mg2+ Cucurbita maxima
4.2.3.19 Mg2+ required Cucurbita maxima
4.2.3.19 Mn2+ can partially replace Mg2+ Cucurbita maxima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 81000
-
 SDS-PAGE, purified enzyme Cucurbita maxima
4.2.3.19 90000
-
 gel filtration Cucurbita maxima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Cucurbita maxima involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Cucurbita maxima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.19 separation of activities A and B achieved by hydrophobic interaction chromatography Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.19 seed
-
 Cucurbita maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.19 0.01
-
 purified enzyme Cucurbita maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 copalyl diphosphate
-
 Cucurbita maxima sandaracopimaradiene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate
-
 Cucurbita maxima ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Cucurbita maxima ent-kaurene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Cucurbita maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.19 6.8 7.5
-
 Cucurbita maxima