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Literature summary extracted from

  • Yamaguchi, S.; Saito, T.; Abe, H.; Yamane, H.; Murofushi, N.; Kamiya, Y.
    Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.) (1996), Plant J., 10, 203-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.19 expressed in Escherichia coli as fusion protein with maltose-binding protein, enzyme exclusively shows EC 4.2.3.19 activity Cucurbita maxima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.19 plastid
-
 Cucurbita maxima 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 81000
-
 SDS-PAGE, native enzyme Cucurbita maxima
4.2.3.19 89000
-
 molecular weight of native enzyme predicted from DNA sequence Cucurbita maxima
4.2.3.19 130000
-
 SDS-PAGE, cloned fusion protein Cucurbita maxima
4.2.3.19 131000
-
 molecular weight of cloned fusion protein predicted from DNA sequence Cucurbita maxima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Cucurbita maxima involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Cucurbita maxima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.19 cloned enzyme from E. coli and native enzyme from endosperm Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.19 seed
-
 Cucurbita maxima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Cucurbita maxima ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Cucurbita maxima ent-kaurene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Cucurbita maxima